ANALYSIS OF MERCAPT-NONMERCAPTALBUMIN BY HPLC ON ANION EXCHANGE COLUMN

Human serum albumin is a mixture of mercaptalbumin which has a sulfhydryl group, and nonmercaptalbumin which has a sulfide with cysteine and glutathion. The fraction of mercaptalbumin decreased in a variety of diseases including chronic renal failure, liver cirrhosis, and etc.. Human serum albumin p...

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Published inJapanese Journal of National Medical Services Vol. 46; no. 7; pp. 500 - 503
Main Authors KITAMURA, Yoshio, IWASHIGE, Syuji, INA, Hidekazu, ITOH, Michiho, KANAZAWA, Sadayuki, YAMAMOTO, Keiji, KATOH, Michio, OKUYAMA, Takumasa, MASUZAWA, Manabu, TAMURA, Kazuya
Format Journal Article
LanguageJapanese
Published Japanese Society of National Medical Services 1992
Subjects
anion exchange column
high-performance liquid chromatography
mercaptalbumin
nonmercaptalbumin
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Summary:Human serum albumin is a mixture of mercaptalbumin which has a sulfhydryl group, and nonmercaptalbumin which has a sulfide with cysteine and glutathion. The fraction of mercaptalbumin decreased in a variety of diseases including chronic renal failure, liver cirrhosis, and etc.. Human serum albumin preparations produced by three companies and serum albumin of healthy volunteers and liver cirrhosis patients were analyzed by HPLC on anion exchange column. The fractions of mercaptalbumin in the albumin preparations varied with lots and the companies. The fraction of mercaptalbumin in liver cirrhosis was significantly lower than that in healthy subjects. These results suggest the clinical usefulness of analysis of serum mercapt-nonmercaptalbumin.
ISSN:0021-1699
1884-8729
DOI:10.11261/iryo1946.46.500