Heparinized small connective tissue graft

• Published in: Jinko Zoki Vol. 16; no. 3; pp. 1466 - 1469
• Main Authors: HASHIMOTO, T., SATO, S., SHIRATA, S., OGA, K., OKA, T., NOISHIKI, Y.
• Format: Journal Article
• Language: Japanese
• Published: JAPANESE SOCIETY FOR ARTIFICIAL ORGANS 1987
• Subjects: Antithrombogenicity; Endothelialization; Heparinization; Polyepoxy compounds; Small connective tissue tube
• ISSN: 0300-0818; 1883-6097
• DOI: 10.11392/jsao1972.16.1466

It is very difficult to achieve long-term patency in artificial vessels with a caliber of less than 4mm. In this experiment, autologous connective tissue grafts (3mm in diameter) provided with an anti-thrombogenic property were interposed at femoral or carotid arteries of dogs, and the patency as well as the morphologicol changes of the vessels were examined. Tefron mesh tubes, 3cm in length, which had been implanted subcutaneously into abdominal wall for 21 days, were treated with 2% protamine solution from inside and Denacol Ex-512 from outside of the tubes to make a cross-like between the two agents. After immersion in 1% heparin solution for 20 minutes, they were interposed with an end to end anastomosis as a substitute for arteries of the same dog. The substituted vessels undergoing a cross-link for 40 minutes showed the longest duration of patency, 13.6±7.2 days on average. In the vessel which was patent for more than 21 days, no thrombus was found in the lumen with newly developed endothelium over 2mm length from the anastrnosed sites, and capillaries were rich in the wall consisting of stratiform structure.