HYBRID TYPE ARTIFICIAL LIVER USING ADULT DOG HEPATOCYTES IN PRIMARY CULTURE

Viable hepatocytes were isolated from adult dog liver by in situ collagenase perfusion, and then cultured on the collagen coated glass plates (w;10cm, 1;20cm) at confluent cell density. Long-stroke type artificial liver module was constructed by piling up 200 glass plates with hepatocytes, containin...

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Published inJinko Zoki Vol. 17; no. 1; pp. 170 - 174
Main Authors FUNATSU, A., HASHIMURA, E., KUMAGAI, F., TSUBURAYA, T., KOMAI, T., KON, T., NAKAMURA, T.
Format Journal Article
LanguageJapanese
Published JAPANESE SOCIETY FOR ARTIFICIAL ORGANS 1988
Subjects
artificial liver
dog hepatocytes
liver functions
primary monolayer culture
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Summary:Viable hepatocytes were isolated from adult dog liver by in situ collagenase perfusion, and then cultured on the collagen coated glass plates (w;10cm, 1;20cm) at confluent cell density. Long-stroke type artificial liver module was constructed by piling up 200 glass plates with hepatocytes, containing 6 billion primary cultured cells in total, i. e. almost equivalent to 1/3 of intact dog liver. All hepatocytes in the module have been quite viable during 2 weeks in perfusion culture, and maintained various liver functions at high level. Moreover, those functions have been maintained for at least 2 weeks in dog plasm as well as in culture medium supplemented with hormones. This hybrid type artificial liver could exert various liver functions like in vivo liver.
ISSN:0300-0818
1883-6097
DOI:10.11392/jsao1972.17.170