ON RNA-POLYMERASES OF LEUKEMIA L 1210 ORIGIN AND AN ENZYMATIC METHOD TO SCREEN ANTITUMOR ANTIBIOTICS

Four DNA-dependent RNA-polymerases were separated from the cell homogenate of mouse Leukemia L1210 cells by DEAE-cellulose column chromatography and tentatively designated as Peaks I, II, III and IV in the elution order. Peak II was inactivated by the addition of α-amanitin and effects of antibiotic...

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Published inJournal of antibiotics Vol. 30; no. 6; pp. 500 - 505
Main Authors SAKANO, KATSU-ICHI, MIZUI, TAKUSHI, AKAGI, KAZUYOSHI, WATANABE, MASAKO, KONDO, HISAO, NAKAMURA, SHOSHIRO
Format Journal Article
LanguageEnglish
Published Japan JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 01.06.1977
Subjects
Amanitins - pharmacology
Animals
Anti-Bacterial Agents - pharmacology
Antibiotics, Antineoplastic - pharmacology
Chromatography, DEAE-Cellulose
DNA-Directed RNA Polymerases - antagonists & inhibitors
DNA-Directed RNA Polymerases - isolation & purification
DNA-Directed RNA Polymerases - metabolism
Drug Evaluation, Preclinical
Hydrogen-Ion Concentration
Hydrolases - pharmacology
Leukemia L1210 - enzymology
Mice
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Summary:Four DNA-dependent RNA-polymerases were separated from the cell homogenate of mouse Leukemia L1210 cells by DEAE-cellulose column chromatography and tentatively designated as Peaks I, II, III and IV in the elution order. Peak II was inactivated by the addition of α-amanitin and effects of antibiotics and enzymes on the RNA-polymerase activity using Peaks I, II and a mixture of Peaks I and II were examined. The RNA-polymerases were used to screen for enzyme inhibitors produced by microbes. This enzymatic method was successfully proved to select antitumor antibiotics.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.30.500