ON RNA-POLYMERASES OF LEUKEMIA L 1210 ORIGIN AND AN ENZYMATIC METHOD TO SCREEN ANTITUMOR ANTIBIOTICS
Four DNA-dependent RNA-polymerases were separated from the cell homogenate of mouse Leukemia L1210 cells by DEAE-cellulose column chromatography and tentatively designated as Peaks I, II, III and IV in the elution order. Peak II was inactivated by the addition of α-amanitin and effects of antibiotic...
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Published in | Journal of antibiotics Vol. 30; no. 6; pp. 500 - 505 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Japan
JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
01.06.1977
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Subjects | |
Online Access | Get full text |
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Summary: | Four DNA-dependent RNA-polymerases were separated from the cell homogenate of mouse Leukemia L1210 cells by DEAE-cellulose column chromatography and tentatively designated as Peaks I, II, III and IV in the elution order. Peak II was inactivated by the addition of α-amanitin and effects of antibiotics and enzymes on the RNA-polymerase activity using Peaks I, II and a mixture of Peaks I and II were examined. The RNA-polymerases were used to screen for enzyme inhibitors produced by microbes. This enzymatic method was successfully proved to select antitumor antibiotics. |
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ISSN: | 0021-8820 1881-1469 |
DOI: | 10.7164/antibiotics.30.500 |