NADPH‐dependent generation of H2O2 in a thyroid particulate fraction requires Ca2
The action of Ca2+ on H2O2 formation catalyzed by an NADPH‐dependent H2O2‐generating system was studied in a thyroid particulate fraction. It was shown that H2O2 formation was inhibited by EGTA and restored in the presence of micromolar concentrations of free Ca2+, and that Mg2+ was unable to restor...
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Published in | FEBS letters Vol. 186; no. 1; pp. 107 - 110 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier
01.07.1985
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Subjects | |
Online Access | Get full text |
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Summary: | The action of Ca2+ on H2O2 formation catalyzed by an NADPH‐dependent H2O2‐generating system was studied in a thyroid particulate fraction. It was shown that H2O2 formation was inhibited by EGTA and restored in the presence of micromolar concentrations of free Ca2+, and that Mg2+ was unable to restore the activity of the EGTA‐inhibited particulate fraction. Consequently, NADPH‐dependent H2O2 formation requires Ca2+, and the Ca2+ stimulation of this process is specific and reversible. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0014-5793 1873-3468 |
DOI: | 10.1016/0014-5793(85)81349-1 |