Microbial diversity in termite nest

In the present study, the microbial diversity of termite nest was studied using bacterial tag encoded amplicon pyrosequencing by both culture-dependent and culture-independent approaches. A total of 10,793 and 4,777 high-quality reads were obtained in culture-independent and culture-dependent approa...

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Bibliographic Details
Published inCurrent science (Bangalore) Vol. 106; no. 10; pp. 1430 - 1434
Main Authors Manjula, A., Sathyavathi, S., Pushpanathan, M., Gunasekaran, P., Rajendhran, J.
Format Journal Article
LanguageEnglish
Published Current Science Association 25.05.2014
Subjects
Actinobacteria
Bacteria
Cultural diversity
Firmicutes
Indian culture
Isoptera
Metagenomics
Proteobacteria
Rarefaction
RESEARCH COMMUNICATIONS
rRNA genes
Sequencing
Species diversity
Termites
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Summary:In the present study, the microbial diversity of termite nest was studied using bacterial tag encoded amplicon pyrosequencing by both culture-dependent and culture-independent approaches. A total of 10,793 and 4,777 high-quality reads were obtained in culture-independent and culture-dependent approaches respectively. The former approach revealed dominant phyla Proteobacteria (32%) and Actinobacteria (20%), whereas the latter approach revealed Firmicutes (74%) and Proteobacteria (22%) as the most dominant phyla. The significant variation in the microbial diversity and composition of termitarium assessed by the two approaches could be due to the fact that culture-dependent approach explored only selected groups of microbial population, whereas metagenomic approach explored complete microbial diversity of termitarium, which provides credence to the application of metagenomic strategy to explore novel microbial species.
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ISSN:0011-3891