RAPID ISOLATION, IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF PECTOBACTERIUM CAROTOVORUM ssp

Thirty-two strains of Pectobacterium carotovorum ssp. (Pec) were isolated from Chinese cabbage and other vegetables. These strains were subject to morphological observation, biochemical and physiological identification as well as 16S rDNA sequence analysis. All strains except D4, Z1-2, Z2-1, Z2-2 an...

Full description

Saved in:
Bibliographic Details
Published inJournal of plant pathology Vol. 92; no. 2; pp. 479 - 483
Main Authors Zhu, L., Xie, H., Chen, S., Ma, R.
Format Journal Article
LanguageEnglish
Published An International Journal of the Italian Phytopathological Society 01.07.2010
Subjects
Biochemistry
Biological taxonomies
Brassica
Cabbages
Fatty acids
Pectobacterium
Pectobacterium carotovorum
Phylogenetics
Phytopathology
Polymerase chain reaction
Ribosomal DNA
Online AccessGet full text

Cover

More Information
Summary:Thirty-two strains of Pectobacterium carotovorum ssp. (Pec) were isolated from Chinese cabbage and other vegetables. These strains were subject to morphological observation, biochemical and physiological identification as well as 16S rDNA sequence analysis. All strains except D4, Z1-2, Z2-1, Z2-2 and Z3-2 manifested the same PCR patterns as indicated by amplifying 16S-23S rDNA ITS and ITS-RFLP. The phylogenetic analysis of the 16S rDNA sequences indicated that these five strains that clustered with Pec strain PCI and close to P. betavasculorum and P. wasabiae were distinct from other strains in the Pec groups. However, the whole-cell fatty acid analysis and the biochemical and physiological characteristics suggested that these five strains could still be identified as Pec. In conclusion, our results suggest that these five strains are probably from a new evolutionary taxonomy oí Pectobacterium carotovorum ssp.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1125-4653
2239-7264