PCR DETECTION AND IDENTIFICATION OF PLANT-PATHOGENIC BACTERIA: UPDATED REVIEW OF PROTOCOLS (1989-2007)

PCR-based methods offer advantages over more traditional diagnostic tests, in that organisms do not need to be cultured prior to their detection and protocols are highly sensitive and rapid. Consequently, there is a shift in research towards DNA-based techniques. Although reports already exist on a...

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Bibliographic Details
Published inJournal of plant pathology Vol. 91; no. 2; pp. 249 - 297
Main Authors Palacio-Bielsa, A., Cambra, M.A., López, M.M.
Format Journal Article
LanguageEnglish
Published An International Journal of the Italian Phytopathological Society 01.07.2009
Subjects
Bacteria
Letter to the Editor
Microbiology
Pathogens
Phytopathology
Plant diseases
Polymerase chain reaction
Product category rules
Pseudomonas
Pseudomonas syringae
Xanthomonas
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Summary:PCR-based methods offer advantages over more traditional diagnostic tests, in that organisms do not need to be cultured prior to their detection and protocols are highly sensitive and rapid. Consequently, there is a shift in research towards DNA-based techniques. Although reports already exist on a variety of PCR-based fingerprinting assays used to analyse the genetic diversity of bacterial populations and define their relationships, this review focuses on the general use of PCR in phytobacteriology for detection and diagnosis purposes. An updated and detailed list of published PCR protocols for detection and identification of plant-pathogenic bacteria is presented and discussed, aimed at facilitating access to information that could be particularly useful for diagnostic laboratories. This compilation includes and discusses 246 articles published between 1989 and 2007 addressing 23 genera, more than 50 species, 10 subspecies and more than 40 pathovars.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1125-4653
2239-7264