Separation of Glycoproteins from Cyst Fluids of Human Ovarian Cystomas in Benign and Their Some Chemical and Serological Properties

• Published in: YAKUGAKU ZASSHI Vol. 113; no. 11; pp. 792 - 802
• Main Authors: HAGIWARA, Teruhiko, OHYAMA, Kunio, YAMAKAWA, Toshio, WATANABE, Hajime, HIRAKAWA, Shun, OHKUMA, Seiichi
• Format: Journal Article
• Language: Japanese
• Published: Japan The Pharmaceutical Society of Japan 01.11.1993
• Subjects: AB-active glycoprotein; Adult; cyst fluid; Cystadenoma, Mucinous - chemistry; Electrophoresis, Polyacrylamide Gel; Female; fucose-rich glycoprotein; Glycoproteins - chemistry; Glycoproteins - isolation & purification; Hemagglutination Inhibition Tests; Humans; ovarian cystoma; Ovarian Cysts - chemistry; Ovarian Neoplasms - chemistry; sialylated T group
• ISSN: 0031-6903; 1347-5231
• DOI: 10.1248/yakushi1947.113.11_792

Perchloric acid-soluble fractions (PASFs 1-3) which were obtained from cyst fluids of two patients [(Nos. 1 (blood group B) and 2 (blood group A))] with ovarian mucinous cystadenoma and one patient (No. 3 (blood group A)) with ovarian dermoid cyst in benign, were identified as glycoproteins by their chemical composition analyses, respectively. Reactivities of PASFs1-3 against blood group anti-sera and lectins suggest that these PASFs did not contain MN blood group substances, Vgu glycoproteins and T-active glycoproteins. PASF 1 was separated into 11 fractions by Sephacryl S-500 gel filtration. From chemical composition, SDS-PAGE and serological property of the main fraction (Fr. 5), other one fraction (Fr. 7) and mild acid hydrolysates of these two fractions, it is possible to presume that PASF 1 consisted of a large number of AB-active and fucose-rich glycoproteins with high molecular weights and some of these glycoproteins bear either NeuNAcα2→6GalNAc (sialyl Tn) group or both sialyl Tn and sialylated T groups.