Effect of Culture Media on Fertilization Rate in Bovine and Caprine Embryos Production

• Published in: International Journal of Environmental and Rural Development Vol. 3; no. 2; p. 56
• Main Authors: BOONKONG, SARUTTIWONG, NAVANUKRAW, CHAINARONG, THAMMASIRI, JIRATTI, JAIKAN, WINAI, URIYAPONGSON, SUTHIPONG
• Format: Journal Article
• Language: English
• Published: Institute of Environmental Rehabilitation and Conservation, Research Center 2012
• Subjects: beef; culture media; embryo; goat; in vitro fertilization
• ISSN: 2185-159X; 2433-3700
• DOI: 10.32115/ijerd.3.2_56

The objective of this experiment was to determine the ef-fect of culture media (Tyrode Albumin Lactate Pyruvate-In Vitro Fertilization-Synthetic Oviduct Fluid; TALP-IVF-SOF and SAGE mediaTM) on fertili-zation rate in bovine and caprine embryos production. Thai crossbred bovine ovaries (n=40) were collected at a local abattoir located in Khon Kaen municipali-ty. Caprine ovaries (n=22) were collected by the surgery method at the Depart-ment of Animal Science, Faculty of Agriculture, Khon Kaen Uni-versity. In order to compare the culture media, oocytes were recovered and ran-domly cultured in TALP-IVF-SOF and SAGE mediaTM during in vitro oocytes maturation and fertilization as described by the standard protocols for in vitro maturation, in vitro fertilization and in vitro culture, respectively. Oocytes, obtained from ovaries with corpus luteum (CL) and without CL, were recovered and determined as recovery rate prior in vitro culture. The results revealed that the recovery rates of bovine and caprine oocytes were not significantly different between ovaries with CL (58.5 and 27.6%) without CL (43.5 and 29.4%) respec-tively; bovine oocytes had significantly more layers of cumulus than caprine oo-cytes. IVF and IVC, developed from two cells to blastocyst stage, derived from SAGE mediaTM were better than that of TALP-IVF-SOF.