培養不可能な状態(VNC)のHelicobbcter pyloriの培養条件の検討
通常培養可能な病原菌が, 生きているにもかかわらず, 通常の培養方法では発育しないViable but nonculturable (VNC )と呼ばれる状態が知られている.coccoid formのH.pyloriはまさにこの状態にあると考えられる.今回, 私たちは, in vitroにおいて, 消化性潰瘍患者から分離された菌株から得られたcoccoidformからの培養を試みた.硫酸アンモニウム溶液中で熱刺激を行った後に, ヒト溶赤血球, 血清および細胞修復剤であるピルビン酸ナトリウムを加えた液体培地中で, cocclid formかららせん状桿菌の発育を認めた.また, この発育菌は血液寒...
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| Published in | 感染症学雑誌 Vol. 73; no. 1; pp. 15 - 19 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
一般社団法人 日本感染症学会
1999
|
| Online Access | Get full text |
| ISSN | 0387-5911 1884-569X |
| DOI | 10.11150/kansenshogakuzasshi1970.73.15 |
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| Abstract | 通常培養可能な病原菌が, 生きているにもかかわらず, 通常の培養方法では発育しないViable but nonculturable (VNC )と呼ばれる状態が知られている.coccoid formのH.pyloriはまさにこの状態にあると考えられる.今回, 私たちは, in vitroにおいて, 消化性潰瘍患者から分離された菌株から得られたcoccoidformからの培養を試みた.硫酸アンモニウム溶液中で熱刺激を行った後に, ヒト溶赤血球, 血清および細胞修復剤であるピルビン酸ナトリウムを加えた液体培地中で, cocclid formかららせん状桿菌の発育を認めた.また, この発育菌は血液寒天培地およびスキロー培地においても発育が可能であった.VNC状態からの培養には, 刺激による休眠状態からの覚醒, さらに覚醒した細胞に対する栄養要求の実現の二つの過程が必要である. |
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| AbstractList | 通常培養可能な病原菌が, 生きているにもかかわらず, 通常の培養方法では発育しないViable but nonculturable (VNC )と呼ばれる状態が知られている.coccoid formのH.pyloriはまさにこの状態にあると考えられる.今回, 私たちは, in vitroにおいて, 消化性潰瘍患者から分離された菌株から得られたcoccoidformからの培養を試みた.硫酸アンモニウム溶液中で熱刺激を行った後に, ヒト溶赤血球, 血清および細胞修復剤であるピルビン酸ナトリウムを加えた液体培地中で, cocclid formかららせん状桿菌の発育を認めた.また, この発育菌は血液寒天培地およびスキロー培地においても発育が可能であった.VNC状態からの培養には, 刺激による休眠状態からの覚醒, さらに覚醒した細胞に対する栄養要求の実現の二つの過程が必要である. |
| Author | 貫名, 正文 田村, 俊秀 下山, 孝 黒川, 学 冨田, 周介 仲西, 寿男 |
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| References | 4) Roszak DB, Clowell RR: Survival strategies of bacteria in the natural environment. Microbiol Rev 1987; 51: 365-379. 13) Wai SN, Moriya T, Kondo K, Misumi H, Amako K: Resuscitation of Vibrio cholerae 0 1 strain TSI-4 from a viable but nonculturable state by heat shock. FEMS Microbiol Lett 1996; 136: 187-191. 8) Nilsson L, Oliver JD, Kjelleberg S: Resuscitation of Vibrio vulnificus from the viable but nonculturable state. J Bacteriol 1991; 173: 5054-5059. 15) Weichart D, Kjelleberg S: Stress resistance and recovery potential of culturable and viable but nonculturable cells of Vibrio vulnificus . Microbiology 1996; 142: 845-853. 17) Wayne LG: Dormant of Mycobacterium tuberculosis and latency of disease. Eur J Clin Microbiol Infect Dis 1994; 13: 908-914. 12) Enroth H, Engstrand L: Immunomagnetic separation and PCR for detection of Helicobacter pylori in water and stool specimens. J Clin Microbiol 1995; 33: 2162-2165. 18) Nilsson L, Oliver JD, Kjelleberg S Resuscitation of Vibrio vulnificus from the viable but nonculturable state. J Bacteriol 1991; 173: 5054-5059. 9) Jones DM, Sutcliffe EM, Curry A: Recovery of viable but nonculturable Campylobacter jejuni . J Gen Microbiol 1991; 137: 2477-2482. 5) Shahamat M, Mai U, Paszko-Kolva C. Kessel M, Clowell RR: Use of autoradiography to assess of viability of Helicobacter pylori in water. Appl Environ Microbiol 1993; 59: 1231-1235. 2) Bode G, Mauch F, Malfertheiner P: The coccoid form of Helicobacter pylori, Criteria for their viability. Epidemiol Infect 1993; 111: 483-490. 14) Welsh J, Mc Clelland M: Characterization of pathogenic microorganisms by genomic fingerprinting using arbitrarily primes PCR. Persing, D. H.(Eds), Diagnostic molecular microbiology, principles and applications. ASM press, Washington D.C., 1993, 595-602. 1) Warren JR, Marshall BJ: Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1983; 321: 1273-1275. 11) 黒川学, 南出正之, 貫名正文, 他: 消化性潰瘍患者大便からのHelicohbcter pylori検出に対するPCR法診断の有用性. 感染症誌 1997; 71: 11681171. 3) Roszak DB, Grimes DJ, Colwell RR: Viable but nonrecoverable stage of Salmonella enteritidis in aquatic systems. Can J Microbiol 1984; 30: 334-338. 7) Xu H-S, Roberts N, Singleton FL, Attwell RW, Grimes DL, Colwell RR: Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microb Ecol 1982; 8: 313-323. 10) Colwell RR, Brayton BR, Grimes DJ, Roszak DB, Huq SA, Palmer LM: Viable but nonculturable Vibrio cholerae and related pathogens in the environment: implications for release of genetically engineered microorganisms. Biotechnol 1985; 3: 817-820. 6) Rollins DM, Clowell WW: Viable but nonculturable state of Campylobacter jejuni and it's role in survival in the natural aquatic environment. Appl Environ Microbiol 1986; 52: 531-538. 16) Colwell RR, Brayton P, Herrington D, Tall B, Huq A, Levine MM: Viable but nonculturable Vibrio cholerae 0 1 revert to a culturable state in the human intestine . World J Microbiol Biotechonol 1996; 12: 28-31. |
| References_xml | – reference: 3) Roszak DB, Grimes DJ, Colwell RR: Viable but nonrecoverable stage of Salmonella enteritidis in aquatic systems. Can J Microbiol 1984; 30: 334-338. – reference: 4) Roszak DB, Clowell RR: Survival strategies of bacteria in the natural environment. Microbiol Rev 1987; 51: 365-379. – reference: 1) Warren JR, Marshall BJ: Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1983; 321: 1273-1275. – reference: 14) Welsh J, Mc Clelland M: Characterization of pathogenic microorganisms by genomic fingerprinting using arbitrarily primes PCR. Persing, D. H.(Eds), Diagnostic molecular microbiology, principles and applications. ASM press, Washington D.C., 1993, 595-602. – reference: 13) Wai SN, Moriya T, Kondo K, Misumi H, Amako K: Resuscitation of Vibrio cholerae 0 1 strain TSI-4 from a viable but nonculturable state by heat shock. FEMS Microbiol Lett 1996; 136: 187-191. – reference: 17) Wayne LG: Dormant of Mycobacterium tuberculosis and latency of disease. Eur J Clin Microbiol Infect Dis 1994; 13: 908-914. – reference: 2) Bode G, Mauch F, Malfertheiner P: The coccoid form of Helicobacter pylori, Criteria for their viability. Epidemiol Infect 1993; 111: 483-490. – reference: 5) Shahamat M, Mai U, Paszko-Kolva C. Kessel M, Clowell RR: Use of autoradiography to assess of viability of Helicobacter pylori in water. Appl Environ Microbiol 1993; 59: 1231-1235. – reference: 6) Rollins DM, Clowell WW: Viable but nonculturable state of Campylobacter jejuni and it's role in survival in the natural aquatic environment. Appl Environ Microbiol 1986; 52: 531-538. – reference: 15) Weichart D, Kjelleberg S: Stress resistance and recovery potential of culturable and viable but nonculturable cells of Vibrio vulnificus . Microbiology 1996; 142: 845-853. – reference: 9) Jones DM, Sutcliffe EM, Curry A: Recovery of viable but nonculturable Campylobacter jejuni . J Gen Microbiol 1991; 137: 2477-2482. – reference: 16) Colwell RR, Brayton P, Herrington D, Tall B, Huq A, Levine MM: Viable but nonculturable Vibrio cholerae 0 1 revert to a culturable state in the human intestine . World J Microbiol Biotechonol 1996; 12: 28-31. – reference: 10) Colwell RR, Brayton BR, Grimes DJ, Roszak DB, Huq SA, Palmer LM: Viable but nonculturable Vibrio cholerae and related pathogens in the environment: implications for release of genetically engineered microorganisms. Biotechnol 1985; 3: 817-820. – reference: 11) 黒川学, 南出正之, 貫名正文, 他: 消化性潰瘍患者大便からのHelicohbcter pylori検出に対するPCR法診断の有用性. 感染症誌 1997; 71: 11681171. – reference: 7) Xu H-S, Roberts N, Singleton FL, Attwell RW, Grimes DL, Colwell RR: Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microb Ecol 1982; 8: 313-323. – reference: 12) Enroth H, Engstrand L: Immunomagnetic separation and PCR for detection of Helicobacter pylori in water and stool specimens. J Clin Microbiol 1995; 33: 2162-2165. – reference: 8) Nilsson L, Oliver JD, Kjelleberg S: Resuscitation of Vibrio vulnificus from the viable but nonculturable state. J Bacteriol 1991; 173: 5054-5059. – reference: 18) Nilsson L, Oliver JD, Kjelleberg S Resuscitation of Vibrio vulnificus from the viable but nonculturable state. J Bacteriol 1991; 173: 5054-5059. |
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| Snippet | 通常培養可能な病原菌が, 生きているにもかかわらず, 通常の培養方法では発育しないViable but nonculturable (VNC )と呼ばれる状態が知られている.coccoid formのH.pyloriはまさにこの状態にあると考えられる.今回, 私たちは, in vitroにおいて,... |
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| Title | 培養不可能な状態(VNC)のHelicobbcter pyloriの培養条件の検討 |
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