Researches on Ergot Fungus Part 24. Production of Riboflavin by An Induced Mutant of Agropyrum-Type Ergot Fungus in Saprophytic Cultures

• Published in: Nippon Nōgeikagaku Kaishi Vol. 28; no. 2; pp. 151 - 156
• Main Authors: ABE, Matazo, KOZU, Yoshiharu
• Format: Journal Article
• Language: English; Japanese
• Published: Japan Society for Bioscience, Biotechnology, and Agrochemistry 1954
• ISSN: 0002-1407; 1883-6844
• DOI: 10.1271/nogeikagaku1924.28.151

It was found that the strongly unaerobic mutant, A 112 U, which was previously illustrated, (1)(2), produces a yellow pigment in considerable amount in saprophytic cultures, and the pig-ment was riboflavin. It was also found that the said mutant produces more riboflavin in surface cultures than in shaking: it produces the pigment under suitable conditions at the rate of 2500γ per 100cc. of the culture filtrate in surface cultures. It was further found that the ordinary strains obtained from various races of natural sclerotia also produce riboflavin under the same conditions at the rate of 300_??_700γ per 100cc. of the same (170_??_430γ per 1 gram of the dried mycelium) in surface cultures, while these ratural sclerotia themselves contain the pigment only at the rate of 6_??_57γ per 1 gram of the dried material. It is obvious, therefore, that ergot fungi produce riboflavin much larger in saprophytic cultures than in nature, though they show marked differences among the races in their ability of producing riboflavin both in nature and in saprophytic cultures.