Altered Serum Lysophosphatidylethanolamine Species Profile in Patients with Autoimmune Pancreatitis

Lysophosphatidylethanolamines (LPEs) are hydrolysis metabolites of polyunsaturated fatty acids and alkenyl group-rich phosphatidylethanolamine (PE). It was reported that LPE functions as a lipid mediator in vivo and it is involved in an inflammatory process of chronic autoimmune disease. LPE is pres...

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Bibliographic Details
Published inTHE SHINSHU MEDICAL JOURNAL Vol. 70; no. 2; pp. 91 - 100
Main Authors YAMAURA, Makoto, HIDAKA, Hiroya
Format Journal Article
LanguageEnglish
Published The Shinshu Medical Society 10.04.2022
Subjects
autoimmune pancreatitis
lysophosphatidylethanolamine
mass spectrometry
plasmalogen
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Summary:Lysophosphatidylethanolamines (LPEs) are hydrolysis metabolites of polyunsaturated fatty acids and alkenyl group-rich phosphatidylethanolamine (PE). It was reported that LPE functions as a lipid mediator in vivo and it is involved in an inflammatory process of chronic autoimmune disease. LPE is present in not only cells but also serum, but the pathophysiological role and metabolic details of serum LPE are not known. In this study, characterization of the serum LPE species in autoimmune pancreatitis (AIP) was performed by mass spectrometry. Serum samples from patients with AIP (n＝7) and healthy subjects (n＝9) were examined for fatty acid compositions using gas chromatography mass spectrometry. LPE species were analyzed using matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Blood lipid tests, cholesterol and triglyceride were almost in the criteria range in patients with AIP. Serum arachidonic acid (C20 : 4) levels were higher in patients with AIP than in healthy subjects, but eicosapentaenoic acid (C20 : 5) and docosahexaenoic acid (C22 : 6) concentrations were not significantly different. In patients with AIP, serum total LPE levels were lower than those in healthy subjects, and LPE 20 : 4 levels were positively correlated with LPE 20 : 5 and LPE 22 : 6 levels. LPE p16 : 0 (plasmalogen-type) level was negatively correlated with LPE 20 : 4, LPE 20 : 5 and LPE 22 : 6 levels. This study showed that though serum lipids and the fatty acid compositions of patients with AIP resembled healthy subjects, serum LPE level of patients were significantly low and some serum LPE species fluctuated in a correlated way.
ISSN:0037-3826
1884-6580
DOI:10.11441/shinshumedj.70.91