Anti-Obesity Effects of Selective Agonists to the β3-Adrenergic Receptor in Dogs. II. Recruitment of Thermogenic Brown Adipocytes and Reduction of Adiposity after Chronic Treatment with a β3-Adrenergic Agonist

• Published in: Journal of Veterinary Medical Science Vol. 60; no. 4; pp. 465 - 469
• Main Authors: SASAKI, Noriyasu, UCHIDA, Eiji, NIIYAMA, Masayoshi, YOSHIDA, Toshihide, SAITO, Masayuki
• Format: Journal Article
• Language: English
• Published: JAPANESE SOCIETY OF VETERINARY SCIENCE 1998
• Subjects: adipose tissue; canine; obesity; uncoupling protein; β3-adrenergic receptor
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.60.465

The aim of this study was to evaluate the effectiveness of β3-adrenergic agonists for the treatment and prevention of obesity in the dog. When a selective β3-adrenergic agonist, CL316, 243 (0.1 mg/kg), was given orally to adult beagles every day for 5-7 weeks, body weight and girth were decreased compared with control placebo-treated dogs. Gross anatomical examinations revealed no noticeable abnormalities in CL316, 243-treated dogs, except an apparent decrease in abdominal fat. Immunohistochemical examination of perirenal adipose tissue showed a remarkable increase in brown adipocytes expressing a thermogenic protein, uncoupling protein (UCP). The increased expression of UCP and its mRNA in CL316, 243-treated dogs was also confirmed by Western blot and reverse transcription polymerase chain reaction analyses. It was concluded that treatment with a β3-adrenergic agonist stimulates UCP expression, which may lead to an increase in energy expenditure, and thereby is useful for the treatment and prevention of obesity in the dog.