マウス脳におけるciliary rootletの形態は領域によって異なるパターンを示す

• Published in: 日本薬理学会年会要旨集 p. 3-B-SS14-4
• Main Authors: 高山, 晃行, 上村, 健士郎, 小山, 奈々, 飯橋, 快斗, 林, 美羽, 肖, 中洋, 中谷, 仁, 澤野, 俊憲, 田中, 秀和
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2023
• Subjects: brain; central nervous system; その他
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.97.0_3-B-SS14-4

Primary cilia are short protrusions that are present in most cells and function as an extracellular environment sensor. In some tissues, a supporting cytoskeleton called the ciliary rootlet (CR) extends from the base of the primary cilium toward the interior of the cell. Dysfunction of primary cilia causes ciliopathies that lead to serious disruption of brain tissue structure and function, but there is no effective treatment for ciliopathies. Recent studies reveal the molecular mechanisms that cause dynamic changes in primary cilia providing the bases for the development of therapeutic drugs. However, CRs in the CNS have not been well studied; their morphology or function are still unknown. Here, we revealed that CRs in C57BL/6 mouse brain are abundant, and their morphology shows distinct pattern in each region. CR morphology in the CNS was roughly classified into three types: linear, circular, and fluffy. The ratio of linear CRs decreased and that of circular CRs increased along with the mouse development. Generally, the CRs were located on the apical dendritic side of cell bodies. Interestingly, there was a positive correlation between CRs length and cilia length, that is, longer cilia are equipped with longer CRs in the brain. The clarification of characteristics of the CRs should lead to a more detailed understanding of the molecular mechanisms of cilia function and to the development of treatments for ciliopathies.