SH-SY5Y細胞におけるAβ誘発タウの過リン酸化に対するエルゴチオネインの抑制効果

• Published in: 日本薬理学会年会要旨集 p. 2-B-P-179
• Main Authors: 柴垣, 郁弥, 小菅, 葉利, 板花, 将輝, 加藤, 優希, 松本, 聡, 中道, 範隆
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2022
• Subjects: amyloid beta-protein; neuronal death; tau protein; その他
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.96.0_2-B-P-179

Ergothioneine (ERGO) is a hydrophilic antioxidant contained in the food and is distributed to the brain after oral intake, exhibiting a neuroprotective effect. ERGO protected PC12 cells against cellular toxicity induced by amyloid beta (Aβ) and improved impairment of learning and memory ability in mice administered with Aβ. However, the effects of ERGO on hyperphosphorylation of tau protein is unclear. In the present study, we investigated whether ERGO suppresses Aβ-induced hyperphosphorylation of tau protein in human neuroblastoma SH-SY5Y cells. SH-SY5Y cells were differentiated using culture medium containing 1% or 10% fetal bovine serum (FBS) with 10 μM retinoic acid. Exposure to Aβ25-35 clearly decreased cellular viability in SH-SY5Y cells differentiated in the 10% FBS medium, but not 1% FBS medium. Pretreatment with ERGO protected SH-SY5Y cells against cellular toxicity induced by Aβ25-35 in a dose-dependent manner. Exposure of SH-SY5Y cells to Aβ25-35 increased expression of phosphorylated tau protein, and the increase was suppressed by pretreatment with ERGO in a dose-dependent manner. These results suggest that ERGO may suppress hyperphosphorylation of tau protein and alleviate neurotoxicity induced by Aβ.