ミクログリアが行動に及ぼす影響について

• Published in: 日本薬理学会年会要旨集 p. 3-B-P-096
• Main Authors: 杉本, 莉菜, 島田, 樹, 中谷, 仁, 澤野, 俊憲, 守村, 直子, 等, 誠司, 田中, 秀和
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2023
• Subjects: behavior; microglia
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.97.0_3-B-P-096

Microglia is a type of glial cell that play a multifaceted role in various functions, including the development and maintenance of neurons, phagocytosis of abnormal or dead cells, pruning of dendritic spine, and spine formation. However, there have been limited reports on the impact of microglia on the behavior of individual organisms. When orally administered the Colony Stimulating Factor 1 Receptor (CSF1R) inhibitor drug PLX3397 (referred to as PLX) to mice, microglia can be temporarily removed from the brain. In this study, we conducted an open field test using PLX to investigate the effects of microglia removal on the behavior of mice.We provided 3-4-month-old C57BL/6 strain mice (both males and females) with food containing 600 ppm of PLX for seven consecutive days and analyzed their total distance traveled, the percentage of time spent in the center, and rearing behavior. The results showed that there were no significant differences in the total distance and the time spent in the center area. However, the microglia removal group tended to spend more time in the center area compared to the control group, and the number of rearing behaviors significantly decreased in the microglia removal group compared to the control group.This study suggests that mice with microglia removal exhibit a reduction in anxiety-like behavior compared to the control group.