遊走する肺胞マクロファージのアクチン細胞骨格制御におけるERMタンパク質の役割

• Published in: 日本薬理学会年会要旨集 p. 1-B-P-060
• Main Authors: 三浦, 綾子, 實松, 史幸, 武谷, 立
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2023
• Subjects: actin; lung
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.97.0_1-B-P-060

The motility of macrophages is essential for the innate immunity system. The efficient movement of macrophages requires close coordination between processes at the leading and trailing edges of the cell. When macrophages sense chemotactic factors, they exhibit a polar morphology that is characterized by the formation of lamellipodia at leading-edge and uropod at the trailing-edge. In the lamellipodia formation, actin filament polymerization produces a protrusive force on the cell membrane that promotes the enlargement of the lamellipodia. In uropod formation, Rho, ROCK and actomyosin bundles, generate forces and maintain contractility posteriorly. The ERM (Ezrin/Radixin/Moesin) proteins are also accumulated to the uropod and link cortical actin-based cytoskeletons to the plasma membrane, thereby regulating morphology and motility. However, the relationship between actin-membrane linkage and actin polymerization is still remain elusive. In addition, previous findings are based on experiments with bone marrow-derived immune cells and it is not known whether similar results can be achieved with tissue-specific macrophages such as alveolar macrophages. In the present study, we prepared alveolar macrophages isolated from bronchoalveolar lavage fluids of mice and analyzed the involvement of ERM and the actin polymerization system in cell migration. Based on our present findings, we will discuss the coupling between actin-membrane linkage and actin polymerization in directed cell migration of alveolar macrophages.