ブラジル産プロポリスに含まれるIL-33遺伝子発現抑制成分としてのベツレトールの単離・同定

• Published in: 日本薬理学会年会要旨集 p. 3-B-P-312
• Main Authors: 水口, 博之, Aurpita, Shaha, Rezwanul, Islam, 北村, 嘉章, 武田, 憲昭, 福井, 裕行
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2022
• Subjects: antiallergic agent; antiinflammatory agent; eosinophil; interleukin
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.96.0_3-B-P-312

IL-33 is involved in the pathogenesis of chronic inflammations through the induction of Th2 cytokines and eosinophils. We showed that IL-33 gene expression level was correlated with the blood eosinophils number of patients with pollinosis. It suggests that suppression of IL-33 gene expression could ameliorate IL-33-induced eosinophilic chronic inflammation. It was reported that Brazilian Green Propolis (BGP) has many pharmacological properties. We showed that BGP suppressed the expression of histamine H1 receptor and IL-9 genes. Here, we sought to investigate the effect of BGP on the suppression of IL-33 gene expression and identify the responsible active compound. The BGP ethanolic extract suppressed PMA-induced IL-33 gene up-regulation in Swiss3T3 cells. It was fractionated using various solvents. The active fraction, then, was further fractionated by silica gel column chromatography, followed by Sephadex LH-20 column. A single compound was detected in TLC, and FTIR and NMR analyses identified the compound as betuletol. Betuletol suppressed PMA-induced IL-33 gene up-regulation. Immunoblot analysis showed that betuletol suppressed PMA-induced ERK phosphorylation. In conclusion, betuletol was identified as an active compound, and it suppresses IL-33 gene expression through the inhibition of ERK phosphorylation.