Detection of HIV-1 proviral DNA and RNA in serum from HIV-1 sero-positive hemophiliacs by polymerase chain reaction

• Published in: Rinshō ketsueki Vol. 34; no. 5; p. 620
• Main Authors: Horikoshi, Y, Ichikawa, M, Tonouchi, T, Mimaya, J, Morita, T, Ohtsuka, Y, Kawabata, H
• Format: Journal Article
• Language: Japanese
• Published: Japan 01.05.1993
• Subjects: Acquired Immunodeficiency Syndrome - microbiology; Adolescent; Adult; Child; DNA, Viral - analysis; Genome, Viral; Hemophilia A - microbiology; HIV Seropositivity - microbiology; HIV-1 - genetics; HIV-1 - isolation & purification; Humans; Male; Middle Aged; Proviruses - genetics; RNA, Viral - analysis
• ISSN: 0485-1439
• DOI: 10.11406/rinketsu.34.620

We amplified HIV-1 proviral DNA and RNA in serum by DNA-PCR and RT-RCR in 20 HIV-1 seropositive hemophiliacs asymptomatic carrier (AC): 10, AIDS-related complex (ARC):8, AIDS:2). HIV-1 DNA sequences were identified in 44 (93.6%) of 47 specimens. 23 (92.0%) of 25 samples in AC, 13 (100%) of 13 in ARC, 8 (88.8%) of 9 in AIDS had detectable bands. RNA sequences were identified in 36 (85.7%) of 42 specimens. 17 (70.8%) of 24 specimens in AC, 13 (100%) of 13 in ARC, 5 (100%) of 5 in AIDS cases had detectable bands. RNA in serum was strongly positive in two asymptomatic patients with progression to AIDS who showed no changes of other markers. Patients with almost normal CD4 cell counts had trace amounts of proviral DNA and RNA in serum. RNA in serum is the parameter most predictive of progression to AIDS and is detectable earlier than other parameters. From the results of RT-PCR before and after seroconversion, the serum of one patient was shown to be positive at 5 months before seroconversion.