Photoregulation of adventitious and axillary shoot proliferation in menthol mint, Mentha arvensis

A direct and indirect methodology for efficient shoot proliferation and regeneration from various expiants of Mentha arvensis has been developed by studying the interactive effect of plant growth regulator and light colour. Nodal expiants cultured on Murashige and Skoog's (MS) medium supplement...

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Bibliographic Details
Published inCurrent science (Bangalore) Vol. 80; no. 7; pp. 878 - 881
Main Authors Bhat, Savithri, Gupta, S. K., Tuli, R., Khanuja, S. P. S., Sharma, S., Bagchi, G. D., Kumar, Anil, Kumar, Sushil
Format Journal Article
LanguageEnglish
Published Current Science Association 10.04.2001
Subjects
Adventitious shoots
Callus
Cytokinins
Light
Optical filters
Plant cells
Plant growth regulators
Plant tissues
Plants
RESEARCH COMMUNICATIONS
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Summary:A direct and indirect methodology for efficient shoot proliferation and regeneration from various expiants of Mentha arvensis has been developed by studying the interactive effect of plant growth regulator and light colour. Nodal expiants cultured on Murashige and Skoog's (MS) medium supplemented with 10 μM thidiazuron and incubated under red light, proliferated an average of 50 shoots after 30 days, whereas internodal explants cultured in modified MS medium supplemented with 40 μM 6-benzylaminopurine and 0.5 μM α-naphthaleneacetic acid and exposed to red light, regenerated an average of 200 shoots after 60 days. Among the six cultivars tested, internodal explants of cv. Gomti regenerated about 90 shoots after 20 days of culture. Under the above conditions, leaf expiants were observed to regenerate an average of 60 shoots after 60 days of culture, which was preceded by callus development. The protocol standardized for high efficiency shoot proliferation and regeneration in M. arvensis from nodal, internodal and leaf expiants is suitable for micropropagation, genetic transformation and for obtaining somaclonal variants in this essential oil-yielding crop plant.
ISSN:0011-3891