Inositol Cyclic Trisphosphate [Inositol 1,2-(cyclic)-4,5-trisphosphate] is Formed upon Thrombin Stimulation of Human Platelets

Cleavage of polyphosphoinositides in vitro by phospholipase C results in formation of both cyclic and noncyclic inositol phosphates. We have now isolated the cyclic product of phosphatidylinositol 4,5-bisphosphate cleavage, inositol 1,2-(cyclic)-4,5-trisphosphate [cIns(1:2,4,5)P3], from thrombin-tre...

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Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 83; no. 17; pp. 6397 - 6401
Main Authors Ishii, Hidemi, Connolly, Thomas M., Bross, Teresa E., Majerus, Philip W.
Format Journal Article
LanguageEnglish
Published National Academy of Sciences of the United States of America 01.09.1986
Subjects
Biochemistry
Elution
Inositol phosphates
Inositols
Phenyls
Phosphates
Phosphatidylinositols
Photoreceptors
Platelets
Radioactive decay
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Summary:Cleavage of polyphosphoinositides in vitro by phospholipase C results in formation of both cyclic and noncyclic inositol phosphates. We have now isolated the cyclic product of phosphatidylinositol 4,5-bisphosphate cleavage, inositol 1,2-(cyclic)-4,5-trisphosphate [cIns(1:2,4,5)P3], from thrombin-treated platelets. We found 0.2-0.4 nmol of cIns- (1:2,4,5)P3 per 109 platelets at 10 sec after thrombin; none was found in unstimulated platelets or in platelets 10 min after thrombin addition. We conclude that cIns(1:2,4,5)P3 is a major product of polyphosphoinositide metabolism in thrombin-stimulated platelets.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.17.6397