Utility of HBsAg measuring system by BLEIA®-1200, a highly sensitive immunoassay system

• Published in: Kanzo Vol. 55; no. 9; pp. 521 - 529
• Main Authors: Yatsuhashi, Hiroshi, Nakamura, Mika, Kugiyama, Yuki, Sasaki, Ryu, Bekki, Shigemune, Hashimoto, Satoru, Bae, Sung-Kwan, Otani, Masashi, Saeki, Akira, Nagaoka, Shinya, Komori, Atsumasa, Abiru, Seigo, Minekawa, Takayuki, Kamiyama, Michinobu, Okuma, Hiroshi
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society of Hepatology 06.10.2014
• Subjects: bioluminescence; BLEIA; HBsAg; hepatitis B; high sensitive immunoassay
• ISSN: 0451-4203; 1881-3593
• DOI: 10.2957/kanzo.55.521

The utility of BLEIA®-1200, an immunoassay system which performs highly sensitive measurement using bioluminescence and its dedicated reagent BLEIA®' Eiken' HBsAg were reviewed. The detection limit was 0.005 IU/ml which was 6 to 10 times higher in sensitivity than the conventional quantitative reagents. The correlation with chemiluminescent immunoassay (CLIA) was favorable with r=0.983, and parallel shifts were seen in treatment progress. In acute hepatitis B infection, BLEIA® showed positive result as long as HBV-DNA was positive even after CLIA became negative. Although one case in CLIA negative non-B hepatitis patients gave a discrepant result in BLEIA®, it was determined as a case of previous HBV infection since HBcAb was positive. This discrepancy may have been due to the difference in reagent sensitivities. BLEIA® reagent' s utility is suggested in screening for HBV infection, early diagnosis in early phase in HBV infection, early detection in de novo hepatitis infection.