A NEW HPLC METHOD FOR THE DETERMINATION OF GLYCATED HEMOGLOBIN: BASIC AND CLINICAL EVALUATION

• Published in: Journal of The Showa Medical Association Vol. 58; no. 4; pp. 336 - 342
• Main Authors: YAMAMOTO, Yoshiko, TSUJI, Masatomi, YAMAMOTO, Noboru, TAKAGI, Yasushi
• Format: Journal Article
• Language: English; Japanese
• Published: The Showa University Society 1998
• Subjects: diabetes mellitus (DM); glycated hemoglobin (HbA1C); high performance liquid chromatography (HPLC); stable hemoglobin A1C (s-A1C)
• ISSN: 0037-4342; 2185-0976
• DOI: 10.14930/jsma1939.58.336

A new HP1C (high performance liquid chromatography) method with a cation-exchange column using non-porous polymer gel for the determination of glycated hemoglobin (HbA1C) was developed. The HP1Ccan clearly separate stable HbA1C (s-A1C) and labile HbA1C (l-A1C) after glucose loading in vivo (OGTT) and in vitro. This HP1C method also shows acceptable precision and reproducibility within 5.0 % of CV. The reference interval, determined for 200 healthy individuals, was 5.22±0.44%. The correlation coefficient between s-A1C and blood sugar, simultaneously determined, was 0.508 (n=75) and two dissociation cases had high l-A 1Cvalues indicating not fasting. Correlation of coefficients between s-A1Cand 1 month or 2 months before determination were 0.509 and 0.467, respectively. Correlation of coefficients of the suspected glucose values from 3 different months (0, 1, 2 months before) had a more significant number of coefficient correlation, r=0.589. A significant correlation was detected between s-bA1Cand fructosamine, r=0.849.