Development of Pathology Specimen Preparation Method by Supercooling Cryopreservation under Magnetic Field Technique of Freezing under Magnetic Field Pathological Diagnosis

• Published in: Academic Collaborations for Sick Children Vol. 5; no. 1; pp. 21 - 27
• Main Authors: Nakagawa, Takashi, Mihara, Makoto, Noguchi, Shuhei, Fujii, Kazuhiro, Ohwada, Tetsuo, Niino, Toyotaro, Sato, Ikuma, Yamashita, Hiromasa, Masamune, Ken, Dohi, Takeyoshi
• Format: Journal Article
• Language: English
• Published: Japan Academic Collaboration Medical Society 2012
• Subjects: Brain tumor; Pathological diagnosis; QOL of childhood; Supercooling freezing; Technique of freezing under magnetic field
• ISSN: 1884-426X; 1884-5002
• DOI: 10.5108/acsc.5.21

Brain tumors have the highest incidence of childhood solid can cers and they alsohave the highest mortality rate among childhood cancers. One fa ctor cited forimproved prognosis in cancer is improvement in surgical extraction rate, but typesof childhood brain tumors are more diverse in comparison to tho se of adults, and itis at present exceedingly difficult for even an experienced pathologist to perform anaccurate diagnosis. We have developed a technique for freezing under magnetic fieldfor the purpose of internal organ cryopreservation, and we cond ucted this study afterconsidering that this freezing technique could be useful for ra pid diagnosis utilizingfrozen tissue during surgery. Results showed the arrangement of neurons to be in muchbetter order with brain tissue frozen under magnetic field than that which was frozenby liquid nitrogen. For pancreatic tissue, it was found that in sulin staining was clearerfor freezing performed under magnetic field than with liquid nitrogen. In short, we foundthat this technique is not simply for the preservation of tissu e, but has the potential toimprove the accuracy of pathological diagnosis and surgical ext raction rate as wellas limiting chemotherapy and radiation therapy. This is the principal outcome that willcontribute to an improved quality of life (QOL) for childhood cancer patients.