Single Particle Analysis of Proteins by Electron Microscopy

Single particle analysis in electron microscopy is a method to reconstruct the three-dimensional images of protein molecules from many projection images. This method enables structural studies of membrane proteins that have been difficult to crystallize. This review summarizes certain principles of...

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Bibliographic Details
Published inButsuri Vol. 57; no. 8; pp. 568 - 574
Main Authors Ueno, Yutaka, Asai, Kiyoshi, Takahashi, Katsutoshi, Sato, Chikara
Format Journal Article
LanguageJapanese
Published The Physical Society of Japan 2002
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Summary:Single particle analysis in electron microscopy is a method to reconstruct the three-dimensional images of protein molecules from many projection images. This method enables structural studies of membrane proteins that have been difficult to crystallize. This review summarizes certain principles of image processing and three dimensional reconstruction used in single particle analysis. Recent results of successful analysis by this method are described, and further improvements and current problems are discussed.
ISSN:0029-0181
2423-8872
DOI:10.11316/butsuri1946.57.568