CLINICAL APPLICATION OF POLYACRYLAMIDE GEL DISC ELECTROPHORETIC ANALYSIS OF SERUM LIPOPROTEINS

• Published in: Japanese Journal of National Medical Services Vol. 48; no. 5; pp. 354 - 359
• Main Authors: MISHIMA, Yasuo, TAKAHASHI, Kiyoshi, ANDO, Mitsuru, KIKUCHI, Miyako, KAMISAKA, Ken, KIBATA, Masayoshi, OKADA, Koji
• Format: Journal Article
• Language: Japanese
• Published: Japanese Society of National Medical Services 1994; 一般社団法人 国立医療学会
• Subjects: coronary risk factor, lipoprotein metabolism; hypertriglyceridemia; PAG electro-phoretic classification; PAG電気泳動型分類; 冠疾患危険因子リポ蛋白代謝; 高中性脂肪血症
• ISSN: 0021-1699; 1884-8729
• DOI: 10.11261/iryo1946.48.354

Hypertriglyceridemia is seemed to be recognized as a key risk factor of coronary heart disease (CHD). To elucidate further risk factors of CHD, it is necessary to assess not only lipid metabolism but also combined risk factors known as syndrome X, and not all of CHD patients have been claimed hyperlipidemia. Therefore, serum cholesterol, triglyceride and HDL cholesterol alone are not sufficient to estimate the whole risk caused by lipoprotein abnormality. Lipoprotein disorders must be examined as metabolic disorders of VLDL and remnant cholesterol. In this study we used the Lipophor system and classified the electrophoretic patterns into four groups according to the variety of shape of LDL portion (S, A, N, and D classification). In normal subjects and patients with old myocardial infarction, diabetes mellitus, we examined the S, A, N, and D classification on their electrophoretic pattern and obtained the high frequency of abnormal electrophoretic patterns such as type N and type D. These abnormal electrophoretic patterns were markedly improved by administration of fibric acid derivative. In conclusion, the Lipophor system is easy to carry out and the classification of electrophoretic pattern is useful to assess the metabolic abnormality of lipoproteins.