Electron and Fluorescence Microscopic Observation of Neuroblastoma

• Published in: Japanese Journal of National Medical Services Vol. 42; no. 5; pp. 385 - 388
• Main Authors: TAKEDA, Takeo, SHIMADA, Masako, NAKADATE, Hisaya, HATAYAMA, Yukiko, HATAE, Yoshio
• Format: Journal Article
• Language: Japanese
• Published: Japanese Society of National Medical Services 1988
• ISSN: 0021-1699; 1884-8729
• DOI: 10.11261/iryo1946.42.385

A tissue of neuroblastoma, which was found by mass screening and extirpated by surgery, was studied using both electron and fluorescence microscopy. Ultrastructural study showed many cored vesicles of about 100mμ in diameter, which contained electron-dense granules with halo around it. Such granules were located mainly in the processes of the tumor cell, and were found often in groups of three to five or in groups of five to many granules. These granules were coincided with those of noradrenalin in their appearances. Another sample from the same specimen was freeze-dried and treated with formaldehyde vapour. Fluorescence microscopic observation displayed that bright fluorescence located in the cytoplasm and also in the processes of the tumor cells. This substance was considered to be noradrenalin judging from the condition of reactions.