Effects of Onpi-To (One of Herb Medicine) on Production of Active Oxygen Species from Neutrophils in Hemodialysis Patients

• Published in: Japanese Journal of Clinical Chemistry Vol. 24; no. 3; pp. 163 - 169
• Main Authors: Ninomiya, Hiroyuki, Saheki, Shuichi, Shinohara, Rikio, Ishiguro, Isao, Tokunaga, Kenji, Takeuchi, Nozomu, Mistuma, Tadamichi, Murase, Mitsuharu, Kanno, Kazuhisa
• Format: Journal Article
• Language: Japanese
• Published: Japan Society of Clinical Chemistry 1995; 一般社団法人 日本臨床化学会
• Subjects: active oxygen productivity; flow cytometry; methylguanidine; onpi-to; SOD; superoxide dismutase; フローサイトメトリー; メチルグアニジン; 活性酸素産生能; 温脾湯
• ISSN: 0370-5633; 2187-4077
• DOI: 10.14921/jscc1971b.24.3_163

Hemodialysis patients (HD) were treated with onpi-to (an herb medicine) administration, and the changes in active oxygen production by neutrophils, and serum superoxide dismutase activities and methylguanidine levels were examined throughout the treatment. The active oxygen production by neutrophils was measured by a flow-cytometric technique or by a cytochrome c reduction method. The active oxygen production was greater in the neutrophils of the HD patients than in normal subjects with or without phorbol myristate acetate (PMA) stimulation. Superoxide dismutase activities and methylguanidine levels in the serum of HD patients were reduced by the treatment. An in vitro experiment using neutrophils from normal subjects showed that onpi-to suppressed stimulated active oxygen production by phorbol myristate acetate, but not by N formyl-methionyl-leucyl-phenylalanine (fMLP) or opsonized zymosan (OZ).