Studies on the Mechanism of Antibiotic-Resistance Acquirement by Enterobacteriaceae I Report: Antibiotic-resistance of E. coli in the intestinal tract

• Published in: Journal of the Japanese Association for Infectious Diseases Vol. 35; no. 1; pp. 9 - 20
• Main Author: WATANABE, Kohei
• Format: Journal Article
• Language: Japanese
• Published: The Japanese Association for Infectious Diseases 20.04.1961
• ISSN: 0021-4817; 1884-5681
• DOI: 10.11552/kansenshogakuzasshi1926.35.9

Antibiotic-resistance of E. coli was measured with 132 patients in the National-Ashikaga Sanatorium and 1, 138 healthy persons of various collective bodies in order to clarify the mechanism of antibiotic-resistance acquirement by enterobacteriaceae. Antibiotic-resistance of E. coli was determined using Mac Conkey medium containing 25 ry/ml of chloramphenicol (CM), streptomycin (SM) and tetracycline (TC). Tuberculosis patients demonstrated in 83.33% and general healthy persons in 32.69% over 25 ry/ml resistance against each one of CM, SM and TC. Over 100 ry/ml resistance against each one of the 3 antibiotics was found in 50.76% of tuberculosis patients and in 17.57% of general healthy persons. In tuberculosis patients SM-single-resistant strains were most frequently encountered (16.67%), followed by two-antibiotics (SM, TC)-resistant strains (13.64%), threeantibiotics-resistant ones (10.67%), TC-single-resistant one (7.58%) and two-antibiotics (CM, SM)-resistant ones (3.03%), respectively. In general healthy persons, resistant strains against various antibiotics were isolated in the following percentage order: TC-single ones in 6.94%, SM-single ones in 6.5%, CM. SM. TC-resistant ones in 2.02%, SM. TC-resistant ones in 1.32%, CM. TC-resistant ones in 0.35% and CM. SM resistant ones in 0.09%. In tuberculosis patients treated with SM, the isolation rate of SM-highly-resistant strains was 56.00%, that of three-antibiotics-resistant ones 12.00%, that of CM. SMresistant ones 2.00%, that of SM. TC-resistant ones 20.00%, SM-single resistant ones 12.00% and TC-single-resistant ones 10.00%, revealing that a high resistance not only against SM but also against TC and CM was acquired by the bacilli. An increasing tendency of the isolation rate of E. coli with a high antibiotic-resistance was recognized in general healthy persons in the year of 1960.