High-Pressure NMR Approaches on Proteins

Pressure and temperature are fundamental physical parameters to control molecular properties and equilibria of systems. However, pressure approaches to life science are still very limited compared with temperature, due probably to their technical difficulties. Here, nuclear magnetic resonance (NMR)...

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Bibliographic Details
Published inThe Review of High Pressure Science and Technology Vol. 27; no. 1; pp. 13 - 17
Main Author KITAHARA, Ryo
Format Journal Article
LanguageJapanese
Published The Japan Society of High Pressure Science and Technology 2017
Subjects
cavity
NMR
nuclear magnetic resonance
oxygen
protein
protein denaturation
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Summary:Pressure and temperature are fundamental physical parameters to control molecular properties and equilibria of systems. However, pressure approaches to life science are still very limited compared with temperature, due probably to their technical difficulties. Here, nuclear magnetic resonance (NMR) approaches combined with hydrostatic pressure and gas pressure are reviewed. Using NMR spectroscopy with variable pressure is a useful method for characterizing conformational fluctuation of proteins. Gas-pressure NMR spectroscopy using molecular oxygen provides a general and highly sensitive method for detecting internal hydrophobic cavities, which might be sources of protein's conformational fluctuation.
ISSN:0917-639X
1348-1940
DOI:10.4131/jshpreview.27.13