Sensitive determination of hydrogen peroxide by FIA using an enhanced luminol chemiluminesence system with a mixed dye-sensitizer

• Published in: BUNSEKI KAGAKU Vol. 41; no. 3; pp. 125 - 133
• Main Authors: ISHII, Mikita, SHIRAI, Masako
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society for Analytical Chemistry 05.03.1992
• Subjects: chemiluminescence enhancement technique using the mixed dye-sensitizer; determination of hydrogen peroxide at ultratrace levels; flow injection method; luminol chemiluminescence system
• ISSN: 0525-1931
• DOI: 10.2116/bunsekikagaku.41.3_125

A technique to enhance chemiluminescence (CL) with a mixed dye-sensitizer (eosin Y/uranine) was applied to the determination of hydrogen peroxide at ultratrace levels using the luminol CL system. The enhancement technique was based on the energy transfer phenomenon between some dye-sensitizers. The proposed single line FIA system was equipped with two sample injectors (Rheodyne, 7125 type) placed on the flow line at a distance of 30 cm from each other; the large one (100 μl) at a distance of 10 cm from the pump was employed for the CL reagent solution and the small one (20μl) at a distance of 20 cm to the detector for the hydrogen peroxide solution (sample). The analytical characteristics were as follows. The lower detection limit: 1 × 10-11M in a 20 Ml injection volume, the relative standard deviation: 1.7% in 10 repeated runs involving a 20 Ml injection of the 1 × 10-8 M hydrogen peroxide soution, the analytical time: ca. 6 s, and the interference: some metallic ions {cobalt(II), copper(II), iron(III) etc.: 1 × 10-6} gave weak interfering signals to the CL response of the 1× 10 -8 M hydrogen peroxide solution. The recovery of hydrogen peroxide in the tap water was almost quantitative.