A Rapid, Simple Determination of Plasma Cyclic AMP

Plasma cyclic AMP content was determined without being extracted, using binding protein obtained from rat liver. EDTA was suitable as an anticoagulant for cyclic AMP estimation. Cyclic AMP further added to EDTA plasma was able to be estimated. The estimated values by plasma dilution were almost the...

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Published inRADIOISOTOPES Vol. 27; no. 5; pp. 251 - 254
Main Authors NAKAHATA, Norimichi, NUMATA, Yoshihiko, OSHIMA, Naokazu
Format Journal Article
LanguageJapanese
Published Japan Radioisotope Association 15.05.1978
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Summary:Plasma cyclic AMP content was determined without being extracted, using binding protein obtained from rat liver. EDTA was suitable as an anticoagulant for cyclic AMP estimation. Cyclic AMP further added to EDTA plasma was able to be estimated. The estimated values by plasma dilution were almost the same as the expected values. It was thought that the direct assay was useful for determination of plasma cyclic AMP. Isoproterenol (50 μg/kg, iv) produced an increase of plasma cyclic AMP level accompanied with a decrease of blood pressure and an increase of heart rate in anesthetized dogs. Cyclic AMP level of peripheral venous plasma was 18.6±1.32 p mole/ml in human (N=25), 21.6±3.04 p mole/ml in dogs (N=7) and 50.6±4.59 p mole/ml in rabbit (N=9) . Plasma cyclic AMP level of rabbit was higher than those of human and dog.
ISSN:0033-8303
1884-4111
DOI:10.3769/radioisotopes.27.5_251