PROCEDURE FOR DETERMINATION OF HCFU AND ITS METABOLITES IN BODY FLUIDS

Studies on the assay procedure for HCFU and its metabolites concentration in body fluids were carried out using Staphylococcus aureus 209 P as the indicator organism, and the following results were obtained. 1) 5-FU was extracted from body fluids with dil-HC1 and then separated from HCFU, CPEFU and...

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Published inCHEMOTHERAPY Vol. 27; no. 5; pp. 778 - 785
Main Authors WATANABE, SHUJI, MORI, KENJI, MISONO, HITOSHI, YOKOYAMA, YOSHITSURU
Format Journal Article
LanguageJapanese
Published Japanese Society of Chemotherapy 25.07.1979
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Summary:Studies on the assay procedure for HCFU and its metabolites concentration in body fluids were carried out using Staphylococcus aureus 209 P as the indicator organism, and the following results were obtained. 1) 5-FU was extracted from body fluids with dil-HC1 and then separated from HCFU, CPEFU and CPRFU using Amberlite XAD-2. The fraction containing HCFU, CPEFU and CPRFU was subjected to hydrolysis so that these compounds were converted to 5-FU. HCFU, CPEFU and CPRFU were determined as a whole on a calculation curve for 5-FU. The lower limit of detection was 0.01 μg/ml. 2) By combination of fractionation technic with Amberlite XAD-2 resin and thin layer chromatography, HCFU, CPEFU and CPRFU in body fluids could be determined separately.
ISSN:0009-3165
1884-5894
DOI:10.11250/chemotherapy1953.27.778