Sphingobacteriumsoli sp. nov., isolated from soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 67; no. 7; pp. 2284 - 2288
• Main Authors: Fu, Yu-Song, Hussain, Firasat, Habib, Neeli, Khan, Inam Ullah, Chu, Xiao, Duan, Yan-Qing, Zhi, Xiao-Yang, Chen, Xing, Li, Wen-Jun
• Format: Journal Article
• Language: English; Japanese
• Published: England 01.07.2017
• Subjects: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids - chemistry; Nucleic Acid Hybridization; Phospholipids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Sphingobacterium - classification; Sphingobacterium - genetics; Sphingobacterium - isolation & purification; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; China
• ISSN: 1466-5034
• DOI: 10.1099/ijsem.0.001946

A Gram-stain-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated YIM X0211T, was isolated from a soil sample of Shiling County, Yunnan Province, south-west China. The new isolate was characterized taxonomically by using a polyphasic approach. The strain grew optimally at 30 °C, at pH 7.0 and with 0-3 % (w/v) NaCl. It was positive for catalase and oxidase but negative for H2S production. Comparative 16S rRNA gene sequence analysis showed that strain YIM X0211T fell within the cluster comprising Sphingobacterium species and clustered with Sphingobacterium mizutaii DSM 11724T (97.93 % similarity). The G+C content of the genomic DNA was 41.2 mol%. The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C15 : 0 2-OH, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The polar lipids consisted of phosphatidylethanolamine, sphingolipid, and several unknown phospholipids or lipids. The DNA-DNA hybridization value between strain YIM X0211T and S. mizutaii DSM 11724T was 42.3±0.4 %, which is below the 70 % limit for species delineation. These chemotaxonomic data supported the affiliation of strain YIM X0211T to the genus Sphingobacterium. Based on the recorded phenotypic and genotypic characteristics, it is determined that the isolate represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium soli sp. nov. is proposed. The type strain is YIM X0211T (=KCTC 42696T=CGMCC 1.15966T).