The Effects of Concentrated Growth Factors Produced from Peripheral Blood on Extraction Wound Healing

• Published in: Journal of Oral Tissue Engineering Vol. 22; no. 1; pp. 15 - 22
• Main Authors: OKAMORI, Daisuke, OKAMURA, Tomoharu, NISHIKAWA, Tetsunari, TOMINAGA, Kazuya, NISHIURA, Aki
• Format: Journal Article
• Language: English; Japanese
• Published: Japanese Association of Regenerative Dentistry 2024
• Subjects: bone regeneration; concentrated growth factors; extraction wound healing
• ISSN: 1348-9623; 1880-0823
• DOI: 10.11223/jarde.22.15

In orthodontic treatment, it is common to perform strategic tooth extraction and move teeth towards the extraction sites. Therefore, the rapid completion of the healing process at the extraction site is important for orthodontic treatment. During the initial stages of extraction wound healing, the formation of blood clots and granulation tissue is observed. Concentrated Growth Factors (CGF), produced by excluding blood cell components from blood, contain fibrin and growth factors, which are thought to promote hemostasis and cell proliferation, potentially shortening the wound healing period. In this study, the effect of CGF on the healing process of extraction wounds was observed by comparing an experimental group with CGF implanted into the extraction wounds of beagle dogs and a control group with no implantation. Four weeks after extraction, in the soft tissue at the center of the extraction wounds of the control group, inflammatory cells, capillaries, and thin, irregular collagen fibers were observed. In the experimental group, compared to the control group, there was an increase in new bone volume and a decrease in inflammatory cells. Twelve weeks after extraction, the extraction wounds in the control group were filled with new bone, but their surface was coarse. In the experimental group, the extraction wounds were filled with bone trabeculae, and the surface was covered with thick, dense bone compared to the control group, and the surface was smooth. Furthermore, in the control group, tall osteoblasts and Tartrate-resistant acid phosphatase-positive giant cells were observed on the surface of the new bone within the extraction wounds at 12 weeks after-extraction, but their presence was reduced in the experimental group. These findings suggest that CGF implantation leads to the early completion of extraction wound healing and that implanting CGF into extraction wounds accelerates the healing process. It is expected that this technology will also be used for convenient removal during orthodontic treatment.