The Disturbance of Regulation of Protein Biosynthesis in Necrotizing Processes of Liver Cells I. The Changes of DNA, RNA and Protein Contents of Liver After CCl4 Administration to Male Mice
Changes of DNA, RNA and protein contents of the liver which are the fundamental macromolecules of cells were determined using CCl4-treated male mice, in order to study the molecular mechanisms of liver cell necrosis. The total DNA content of the liver reached a minimum value at 24 hours after the CC...
Saved in:
| Published in | Kanzo Vol. 17; no. 1; pp. 1 - 6 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Japanese |
| Published |
The Japan Society of Hepatology
25.01.1976
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0451-4203 1881-3593 |
| DOI | 10.2957/kanzo.17.1 |
Cover
| Abstract | Changes of DNA, RNA and protein contents of the liver which are the fundamental macromolecules of cells were determined using CCl4-treated male mice, in order to study the molecular mechanisms of liver cell necrosis. The total DNA content of the liver reached a minimum value at 24 hours after the CCl4 administration, and then gradually increased till 72 hours when the value was higher than that at 0 hour. It was postulated that these changes of the total DNA content depended upon either decrease or increase of cells induced by necrosis or regeneration, respectively. The RNA content of the liver per mg DNA also reached a minimum value at 24 hours after the treatment and then increased till 72 hours when the value, however, was lower than that at 0 hour. On the other hand, the protein content of the liver per mg DNA did not varied for the first 24 hours, and then progressively decreased till 72 hours. It was discussed that the regulation of protein biosynthesis of the liver was severely disturbed by the CCl4 administration, and that this disturbance was one of direct factors that caused liver cell necrosis. |
|---|---|
| AbstractList | Changes of DNA, RNA and protein contents of the liver which are the fundamental macromolecules of cells were determined using CCl4-treated male mice, in order to study the molecular mechanisms of liver cell necrosis. The total DNA content of the liver reached a minimum value at 24 hours after the CCl4 administration, and then gradually increased till 72 hours when the value was higher than that at 0 hour. It was postulated that these changes of the total DNA content depended upon either decrease or increase of cells induced by necrosis or regeneration, respectively. The RNA content of the liver per mg DNA also reached a minimum value at 24 hours after the treatment and then increased till 72 hours when the value, however, was lower than that at 0 hour. On the other hand, the protein content of the liver per mg DNA did not varied for the first 24 hours, and then progressively decreased till 72 hours. It was discussed that the regulation of protein biosynthesis of the liver was severely disturbed by the CCl4 administration, and that this disturbance was one of direct factors that caused liver cell necrosis. |
| Author | ARIMASA, Naomichi |
| Author_xml | – sequence: 1 fullname: ARIMASA, Naomichi organization: The First Department of Internal Medicine, Okayama University Medical School |
| BookMark | eNo9UN1OwjAYbQwmInLjE-wFht_X0rW71KloQtQYvDRL132D4uxMO0zg6QUl3pyTk_Nzcc7ZwHeeGLtEmPBcqqsP43fdBNUET9gQtcZUyFwM2BCmEtMpB3HGxjG6CoBnCvKcD9n7YkXJrYv9JlTGW0q6Jnml5aY1vev8Qb2ErifnkxvXxa3vVxRdTPb6iezecTvnl4eMpRgpHgpz900hKaht4wU7bUwbaXzkEXu7v1sUD-n8efZYXM_TNaLyaa0zqSXwOjda8ppMBlSbRoiqIqwbBFWRVgKU4MJCZTRwbqXNFNUK9xExYsXf7jr2ZknlV3CfJmxLE3pnWyp_n8E8gxJViUf4d-3KhJK8-AE4g2UB |
| ContentType | Journal Article |
| Copyright | The Japan Society of Hepatology |
| Copyright_xml | – notice: The Japan Society of Hepatology |
| DOI | 10.2957/kanzo.17.1 |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1881-3593 |
| EndPage | 6 |
| ExternalDocumentID | article_kanzo1960_17_1_17_1_1_article_char_en |
| GroupedDBID | ALMA_UNASSIGNED_HOLDINGS CS3 JSF KQ8 P2P RJT |
| ID | FETCH-LOGICAL-j117n-d8658502d9a852dea60edaf33bbe1df107be87307323c0ba8022c5c67ed71be13 |
| ISSN | 0451-4203 |
| IngestDate | Wed Sep 03 05:59:39 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Language | Japanese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-j117n-d8658502d9a852dea60edaf33bbe1df107be87307323c0ba8022c5c67ed71be13 |
| OpenAccessLink | https://www.jstage.jst.go.jp/article/kanzo1960/17/1/17_1_1/_article/-char/en |
| PageCount | 6 |
| ParticipantIDs | jstage_primary_article_kanzo1960_17_1_17_1_1_article_char_en |
| PublicationCentury | 1900 |
| PublicationDate | 1976/01/25 |
| PublicationDateYYYYMMDD | 1976-01-25 |
| PublicationDate_xml | – month: 01 year: 1976 text: 1976/01/25 day: 25 |
| PublicationDecade | 1970 |
| PublicationTitle | Kanzo |
| PublicationTitleAlternate | Kanzo |
| PublicationYear | 1976 |
| Publisher | The Japan Society of Hepatology |
| Publisher_xml | – name: The Japan Society of Hepatology |
| References | 3) Lowry, O.H.: Protein measurement with the Folin phenol reagent. J. Biol. chem., 193: 265, 1951. 10) Paul, D.: Stimulation of DNA and protein synthesis in fetal-rat liver cells by serum from partially hepatectomized rats. Proc. Nat. Acad. Sic. USA, 69: 374, 1972. 16) 有正修道:肝細胞壊死発生過程における蛋白合成調節機構の異常.II.四塩化炭素投与によるマウスの肝細胞リボゾームの減少とポリゾームの解重合現象.肝臓,17: 7, 1976. 6) Slater, T.F. & Sawyer, B.C.: The stimulatory effects of carbon tetrachloride and other halogenoalkanes on peroxidative reactions in rat liver fractions in vitro. Biochem. J., 123: 805, 1971. 4) Vendrely, R., In Chargaff, E. & Davidson, J.N. (eds.): The Nucleic Acid, vol. 2, Academic Press, New York, 1955, p.155. 13) Yokoi, O.: Deoxyribonucleic acid content of the regenerative nodule cells in liver cirrhosis. 1. Observations on the regenerative nodule cells in an experimental liver cirrhosis. Gastroenterologia Japonica, 2: 1, 1967. 9) Leevy, C.M.: Liver regeneration in experimental carbon tetrachloride intoxication. Proc. Soc. Exptl. Biol. Med., 102: 672, 1959. 11) Lynch, W.E. & Lieberman, I.: A DNA polymerase in liver nuclei whose activity rises with DNA synthesis after partial hepatectomy. Biochem. Biophys. Res. Commun., 52: 843, 1973. 15) Afimasa, N. & Kochakian, C.D.: Epitestosterone and 5α-androstane-3α, 17β-diol: the characteristic metabolites of androst-4-ene-3, 17-dione produced by mouse kidney in vitro. Endocrinology, 92: 72, 1973. 19) 有正修道:肝細胞壊死発生過程における蛋白合成調節機構の異常.III.四塩化炭素投与によるマウス肝細胞の翻訳過程における蛋白合成系因子の活性低下.肝臓,17: 13, 1976. 7) 那谷賢治:諸種化学物質中毒時のマウス肝小葉内に於ける障害部位と毛細血管の態度との関係についての生体顕微鏡的研究.第一篇.肝小葉中心帯を障害する四塩化炭素,クロロフォルム中毒時のマウス肝小葉の生体顕微鏡的研究.日本消化機病学会雑誌,55: 859, 1958. 2) Munro, H.N. & Fleck, A., In Glick, D. (ed.): Methods of Biochemical Analysis, vol. 14, John Wiley and Sons, New York, 1966, p.113. 17) Lee, S.C. & Dahmus, M.E.: Stimulation of eukaryotic DNA-dependent RNA polymerase by protein factors. Proc. Nat. Acad. Sci. USA, 70: 1383, 1973. 14) Arimasa, N. & Kochakian, C.D.: Subcellular localization of the C19-steroid reductase activities of female rat liver. Steroids, 19: 325, 1972. 12) Morley, C.G.D. & Kingdon, H.S.: The regulation of cell growth. 1. Identification and partial characterization of a DNA synthesis stimulating factor from the serum of partially hepatectomized rats. Biochim. Biophys. Acta, 308: 260, 1973. 1) Burton, K., In Colowick, S.P. & Kaplan, N.O. (eds.): Methods in Enzymology, vol. 12, Academic Press, New York, 1968, p.163. 5) Bucher, N.L.R. & Malt, R.A.: Regeneration of Liver and Kidney, Little, Brown and Company, Boston, 1971, P.42. 18) Sarma, D.S.R.: Studies on the nature of attachment of ribosomes to membranes in liver. 1. Influence of ethionine, sparsomycin, CCl4, and puromycin on membrane-bound polyribosomal disaggregation and on detachment of membrane-bound ribosomes from membranes. Lab. Invest., 27: 39, 1972. 8) Morrison, G.R.: Qualitative analysis of regenerating and degenerating areas within the lobule of the carbon tetrachloride-injured liver. Arch. Biochem. Biophys., 111: 448, 1965. |
| References_xml | – reference: 6) Slater, T.F. & Sawyer, B.C.: The stimulatory effects of carbon tetrachloride and other halogenoalkanes on peroxidative reactions in rat liver fractions in vitro. Biochem. J., 123: 805, 1971. – reference: 11) Lynch, W.E. & Lieberman, I.: A DNA polymerase in liver nuclei whose activity rises with DNA synthesis after partial hepatectomy. Biochem. Biophys. Res. Commun., 52: 843, 1973. – reference: 3) Lowry, O.H.: Protein measurement with the Folin phenol reagent. J. Biol. chem., 193: 265, 1951. – reference: 1) Burton, K., In Colowick, S.P. & Kaplan, N.O. (eds.): Methods in Enzymology, vol. 12, Academic Press, New York, 1968, p.163. – reference: 5) Bucher, N.L.R. & Malt, R.A.: Regeneration of Liver and Kidney, Little, Brown and Company, Boston, 1971, P.42. – reference: 8) Morrison, G.R.: Qualitative analysis of regenerating and degenerating areas within the lobule of the carbon tetrachloride-injured liver. Arch. Biochem. Biophys., 111: 448, 1965. – reference: 15) Afimasa, N. & Kochakian, C.D.: Epitestosterone and 5α-androstane-3α, 17β-diol: the characteristic metabolites of androst-4-ene-3, 17-dione produced by mouse kidney in vitro. Endocrinology, 92: 72, 1973. – reference: 7) 那谷賢治:諸種化学物質中毒時のマウス肝小葉内に於ける障害部位と毛細血管の態度との関係についての生体顕微鏡的研究.第一篇.肝小葉中心帯を障害する四塩化炭素,クロロフォルム中毒時のマウス肝小葉の生体顕微鏡的研究.日本消化機病学会雑誌,55: 859, 1958. – reference: 14) Arimasa, N. & Kochakian, C.D.: Subcellular localization of the C19-steroid reductase activities of female rat liver. Steroids, 19: 325, 1972. – reference: 17) Lee, S.C. & Dahmus, M.E.: Stimulation of eukaryotic DNA-dependent RNA polymerase by protein factors. Proc. Nat. Acad. Sci. USA, 70: 1383, 1973. – reference: 13) Yokoi, O.: Deoxyribonucleic acid content of the regenerative nodule cells in liver cirrhosis. 1. Observations on the regenerative nodule cells in an experimental liver cirrhosis. Gastroenterologia Japonica, 2: 1, 1967. – reference: 16) 有正修道:肝細胞壊死発生過程における蛋白合成調節機構の異常.II.四塩化炭素投与によるマウスの肝細胞リボゾームの減少とポリゾームの解重合現象.肝臓,17: 7, 1976. – reference: 9) Leevy, C.M.: Liver regeneration in experimental carbon tetrachloride intoxication. Proc. Soc. Exptl. Biol. Med., 102: 672, 1959. – reference: 12) Morley, C.G.D. & Kingdon, H.S.: The regulation of cell growth. 1. Identification and partial characterization of a DNA synthesis stimulating factor from the serum of partially hepatectomized rats. Biochim. Biophys. Acta, 308: 260, 1973. – reference: 2) Munro, H.N. & Fleck, A., In Glick, D. (ed.): Methods of Biochemical Analysis, vol. 14, John Wiley and Sons, New York, 1966, p.113. – reference: 19) 有正修道:肝細胞壊死発生過程における蛋白合成調節機構の異常.III.四塩化炭素投与によるマウス肝細胞の翻訳過程における蛋白合成系因子の活性低下.肝臓,17: 13, 1976. – reference: 4) Vendrely, R., In Chargaff, E. & Davidson, J.N. (eds.): The Nucleic Acid, vol. 2, Academic Press, New York, 1955, p.155. – reference: 18) Sarma, D.S.R.: Studies on the nature of attachment of ribosomes to membranes in liver. 1. Influence of ethionine, sparsomycin, CCl4, and puromycin on membrane-bound polyribosomal disaggregation and on detachment of membrane-bound ribosomes from membranes. Lab. Invest., 27: 39, 1972. – reference: 10) Paul, D.: Stimulation of DNA and protein synthesis in fetal-rat liver cells by serum from partially hepatectomized rats. Proc. Nat. Acad. Sic. USA, 69: 374, 1972. |
| SSID | ssib002670992 ssib000940394 ssib002670224 ssib058493441 ssj0069113 ssib005879686 ssib002484534 |
| Score | 1.2505639 |
| Snippet | Changes of DNA, RNA and protein contents of the liver which are the fundamental macromolecules of cells were determined using CCl4-treated male mice, in order... |
| SourceID | jstage |
| SourceType | Publisher |
| StartPage | 1 |
| SubjectTerms | DNA RNA |
| Subtitle | I. The Changes of DNA, RNA and Protein Contents of Liver After CCl4 Administration to Male Mice |
| Title | The Disturbance of Regulation of Protein Biosynthesis in Necrotizing Processes of Liver Cells |
| URI | https://www.jstage.jst.go.jp/article/kanzo1960/17/1/17_1_1/_article/-char/en |
| Volume | 17 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | Kanzo, 1976/01/25, Vol.17(1), pp.1-6 |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELZKkRAXxFNAKfKhN5QljuPYlrisSlGh3Ur0IfWCIjtxYJcqW7Xpgf56ZuxsYkQPtFysdR4r2fNl_I09D0K2UmF0peAD5IVGA8XJxLqMJ8bZopYO7lW4oT87KHZP8i-n4nQsl-qjSzo7qa5vjCu5i1ThGsgVo2RvIdnhT-EC_Ab5QgsShvafZfwR5HR1Yb3nv08s8r0vyOW92zALw7zFipOXv1rgeph-BPoHQBaX3fwa9wn6UIGQfHYf3TTebbuzs8uYtu4Bg1wO2Dj8PJseTYNuxqDmH_Nx74AB8cC9gxBnPBmiz2BNbgcXUTw8gGWwG7f0gyrKBQgyS4MqckFVKsUSLkJ9w0GXyr8wExQji1bY4ibVnWlfb_gnjmfC5ISNC9TgNthPdOkfAtUB9ossWd-Uq7sYrgbouEfuZ1L6o_u9rxHl1HnKY5M2V7ngsUkqkdb80dfRybNQUhdjyj3gb5p7ShlWf0B8KMm9mrGQEhdH934cG9CdBZD_leOg5zLHj8mj3gih0zCUJ2RtYZ6SB7PezeIZ-QYioxGw6LKhI7Cw1wOLxsCi0I-ARQdg4QseWNQD6zk5-bRzvL2b9HU4kgVjsk1qBTRVpFmtjRJZ7UyRuto0nFvrWN2wVFqnJC4WGa9SazB6uxJVIV0tGTzCX5D1dtm6l4TWVQWUvgGbmzd5LoxVzBgtrSoabrNcvCIfwsSU5yHZSnkrmb_-v9c3yMPxK3lD1ruLK7cJnLOzbz2IfgNEh4Ff |
| linkProvider | Colorado Alliance of Research Libraries |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+Disturbance+of+Regulation+of+Protein+Biosynthesis+in+Necrotizing+Processes+of+Liver+Cells&rft.jtitle=Kanzo&rft.au=ARIMASA%2C+Naomichi&rft.date=1976-01-25&rft.pub=The+Japan+Society+of+Hepatology&rft.issn=0451-4203&rft.eissn=1881-3593&rft.volume=17&rft.issue=1&rft.spage=1&rft.epage=6&rft_id=info:doi/10.2957%2Fkanzo.17.1&rft.externalDocID=article_kanzo1960_17_1_17_1_1_article_char_en |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0451-4203&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0451-4203&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0451-4203&client=summon |