The Disturbance of Regulation of Protein Biosynthesis in Necrotizing Processes of Liver Cells I. The Changes of DNA, RNA and Protein Contents of Liver After CCl4 Administration to Male Mice

• Published in: Kanzo Vol. 17; no. 1; pp. 1 - 6
• Main Author: ARIMASA, Naomichi
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society of Hepatology 25.01.1976
• Subjects: DNA; RNA
• ISSN: 0451-4203; 1881-3593
• DOI: 10.2957/kanzo.17.1

Changes of DNA, RNA and protein contents of the liver which are the fundamental macromolecules of cells were determined using CCl4-treated male mice, in order to study the molecular mechanisms of liver cell necrosis. The total DNA content of the liver reached a minimum value at 24 hours after the CCl4 administration, and then gradually increased till 72 hours when the value was higher than that at 0 hour. It was postulated that these changes of the total DNA content depended upon either decrease or increase of cells induced by necrosis or regeneration, respectively. The RNA content of the liver per mg DNA also reached a minimum value at 24 hours after the treatment and then increased till 72 hours when the value, however, was lower than that at 0 hour. On the other hand, the protein content of the liver per mg DNA did not varied for the first 24 hours, and then progressively decreased till 72 hours. It was discussed that the regulation of protein biosynthesis of the liver was severely disturbed by the CCl4 administration, and that this disturbance was one of direct factors that caused liver cell necrosis.