Effects of Gonadotropins and Estradiol Added into Maturation Medium of Bovine Oocytes in Vitro on the Subsequent Capacity for Fertilization and Embryonic Development

• Published in: The Japanese journal of animal reproduction Vol. 37; no. 2; pp. 127 - 132
• Main Authors: FUKUSHIMA, Moriyuki, SHIOYA, Yasuo, KUWAYAMA, Masashige, IWASAKI, Setsuo, HANADA, Akira
• Format: Journal Article
• Language: Japanese
• Published: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 25.06.1991
• Subjects: BOVINE OOCYTE MATURATION; GTH AND E2 FOR BOVINE OOCYTES; IN VITRO FERTILIZATION
• ISSN: 0385-9932
• DOI: 10.1262/jrd1977.37.127

Effects of gonadotropins (10 μg/ml LH and 2 or 10 μg/ml FSH) and estradiol-17β (E2:2 μg/ml) added into a maturation medium of bovine oocytes were evaluated for the subsequent capacity of in vitro fertilization and embryonic development into blastocysts after culturing in therabbit oviducts. Immature bovine oocytes collected from follicles of 25 mm in diameter and enclosed with cumulus cells were cultured in vitro for 22 hr in TCM-199 medium containing 25 mM HEPES, 10%(V/V)fetal calf serum, antibiotics and hormones. The combinations of the hormones added to the medium were as follows; (A) none, (B) LH+E2, (C) FSH (2 μg/ml/)+LH+E2, and (D) FSH(10 μg/ml)+LH+E2. The fertilization rates and the developmental rates of 4-cell embryos into blastocysts were not significantly affected by the addition of hormones. The fertilization rates and the developmental rates into blastocysts were: 90.9%(40/44) and 39.6%(40/101) for group A, and 90.7%(39/43) and 34.7%(26/75) for group D. The present study indicates that the addition of hormones to a maturation medium has no positive effect on the capacity for fertilization and embryonic development of bovine oocytes matured in vitro.