The effect of in vivo ultraviolet-B radiation to the activity of mouse macrophage measured by NO2- production Comparision with TNF alpha

• Published in: Ensho Saisei Vol. 21; no. 1; pp. 79 - 83
• Main Authors: Kura, Fumiaki, Yamamoto, Kiichi, Miyoshi-Koshio, Toshiko
• Format: Journal Article
• Language: Japanese
• Published: The Japanese Society of Inflammation and Regeneration 28.01.2001
• Subjects: LPS; macrophage; NO2; TNF alpha; ultraviolet-B
• ISSN: 1346-8022; 1880-5795
• DOI: 10.2492/jsir.21.79

To evaluate the effects of ultraviolet-B (UV-B) light for the immune systems, we examined whether UV-B affect to macrophage (Mφ) functions or not. In this study, as an indicator for activation of macrophages induced from UV-B light irradiated mouse, we investigated two Mφ products, radical NO (NO2-), and TNFα. NO2- produced by stimulation of lipopolysaccaride (LPS) from macrophage was extensively inhibitted by UV-B irradiation to mice from where Mφ were collected. The inhibition did not depend on the LPS concentration, but it was apparently inhibitted even at high amounts of LPS as well as at low dose one. When TNFα was determined as indicator of activity, the degree of inhibition was various. In an exceptional case, although NO2- was extensively inhibited but the inhibition of TNFα was moderate.