Immunological studies on protein precipitating factors in boar semen

• Published in: The Japanese journal of animal reproduction Vol. 23; no. 4; pp. 142 - 147
• Main Authors: MASUDA, Hiroshi, SOEJIMA, Akihiko, ABE, Tsuneo
• Format: Journal Article
• Language: Japanese
• Published: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 30.12.1977
• ISSN: 0385-9932
• DOI: 10.1262/jrd1977.23.142

This experiment was carried out to clarify some properties of protein precipitating factors in boar semen by agar gel diffusion method and to determine whether precipitation reaction was caused by immunoreaction or not. The results obtained are as follows; 1. Boar seminal plasma having protein precipitating factors reacted sharply with 10% egg yolk solution and with autologus, homologus or heterologus serum, but not with the seminal plasma from other animal species, by Ouchterlony's agar gel diffusion method. 2. The precipitating factors were absorbed by washed bull and buck spermatozoa and also by bull spermatozoa heated at 100°C for 10 minutes. The red cells and these ghosts also absorbed the precipitating factors. The precipitating activity of boar seminal plasma did not disappear by heating at 70°C for 30 minutes, but the activity of pig serum antibodies was disappeared by heating at 70°C for 10 minutes. 3. The precipitation of boar seminal plasma to egg yolk solution was inhibited or tended to be inhibited in the presence of bull and buck seminai plasma, but not in the presence of that of cock, depending on the quantity of the plasma. 4. The serum from the boar precipitation positive semen did not develop precipitin line against egg yolk solution. The precipitating factors were not absorbed by antiserum to normal pig serum or by antiserum to pig IgG. The egg yolk solution or serum, heated at 100°C for 10 minutes, reacted with boar seminal plasma. From the above results, it may be concluded that the precipitation of boar seminal plasma to egg yolk solution or serum is not due to the immunoreaction.