Studies on Enzyme Preparations. XX : Studies on Bile Sensitive Lipase. (No. 1) : The Effect of Bile and Bile Acid Salt on Lipase Produced from Mucor and Pseudomonas Species

• Published in: Eisei kagaku Vol. 13; no. 5; pp. 257 - 264
• Main Authors: Ogiso, Taro, Sugiura, Mamoru, Asano, Hiroshi, Nagase, Keizo, Narita, Shuichi
• Format: Journal Article
• Language: Japanese
• Published: The Pharmaceutical Society of Japan 30.10.1967
• ISSN: 0013-273X
• DOI: 10.1248/jhs1956.13.257

The effect of bile juice and bile acid on various microbial lipase prepartions from Aspergillus, Candida Phizopus, Mucor and Pseudomonas were measured. The lipase from Mucor as well as pancreatic lipase was remarkably activated with bile juice (human, cow, pig) and bile acid in vegetable oils and higher triglyceride substrate, but inhibited with sodium cholate in lower triglyceride, such as tripropionin and tributyrin. The lipases from Pseudomonas and Bacillus were slightly activated with sodium cholate and sodium taurocholate, but inhibited with various bile juice at ten times dilution. The lipases from Aspergillus, Candida, and Rhizopus were remarkably inhibited with various bile juice and bile acid. From the result that Mucor lipase was activated with bile juice in various substrates, the lipase seems to exhibit the excellent lipase activities in intestinal canal.