STUDIES on HYBRID ARTIFICIAL LIVER METABOLIC ACTIVITY of ISOLATED HEPATOCYTES after CRYOPRESERVATION

The present experiment was designed to study whether isolated hepatocytes maintained their metabolic activity after cryopreservation. Isolated hepatocytes were obtained from rabbit and prepared by the collagenase digestive methods. The hepatocytes were preserved in a liquid nitrogen freezer at -196°...

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Published inJinko Zoki Vol. 9; no. 2; pp. 390 - 393
Main Authors KASAI, S., OHNO, M., ASAKAWA, M., OIKAWA, I., SEKIGUCHI, S., MITO, M.
Format Journal Article
LanguageJapanese
Published JAPANESE SOCIETY FOR ARTIFICIAL ORGANS 15.04.1980
Subjects
cryopreservation
hybrid artificial liver
isolated hepatocytes
metabolic activity
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Summary:The present experiment was designed to study whether isolated hepatocytes maintained their metabolic activity after cryopreservation. Isolated hepatocytes were obtained from rabbit and prepared by the collagenase digestive methods. The hepatocytes were preserved in a liquid nitrogen freezer at -196°C. The metabolic activity was examined by the incubation technique utilizing a shaker flask. After thawing, the recovery rate of hepatocytes was 50% and their viability was 35%. Some metabolic activities were observed even after storage at -196°C for two weeks. This supports the possibility of developing a hybrid artificial liver utilizing cryopreserved hepatocytes.
ISSN:0300-0818
1883-6097
DOI:10.11392/jsao1972.9.390