A simple and rapid microtechnique for studying a luminol-dependent chemiluminescence of whole blood

• Published in: Ensho Vol. 5; no. 3; pp. 205 - 210
• Main Authors: Kohashi, Osamu, Kohashi, Yukiko, Toyoshima, Shizue, Shigematsu, Nobuaki
• Format: Journal Article
• Language: Japanese
• Published: The Japanese Society of Inflammation and Regeneration 01.07.1985
• ISSN: 0389-4290; 1884-4006
• DOI: 10.2492/jsir1981.5.205

A simple and rapid microtechnique using 10 ul of whole blood was developed for studying a luminol-dependent chemiluminescence (CL) response. This assay have been thought to be unreliable because of poor reproducibility. In fact, the CL response of this assay was intrinsically affected by contamination of red blood cells or other cellular components and plasma constitutents. More importantly, there were several serious problems to get a reliable and reproducible measurement, because this CL respons by itself was varied hours by hours and day by day and different machines gave us a significantly different measurement to the CL response of the same blood sample. We carefully studied the effect of red blood cells and serum on CL response and found that if 10 ul of whole blood were used instead of 100 ul, this assay can be successfully and reliably used for evaluating the phagocytic function of peripheral blood phagocytes, especially by a simultaneous measurement of CL respons against PMA and OZ immediately after bleeding at a certain fixed time of a patient. This simultaneous microtechnique of whole blood CL response will offer a very important knowledge of cell surface functions (FcR and C3bR) of phagocytic cells, involved in the pathological events of various diseases.