Microarray analysis in nasal epithelial cells that are related with Japanese cedar pollinosis

• Published in: Journal of Japan Society of Immunology & Allergology in Otolaryngology Vol. 29; no. 3; pp. 201 - 207
• Main Authors: Fujieda, Shigeharu, Imoto, Yoshimasa
• Format: Journal Article
• Language: Japanese
• Published: JAPAN SOCIETY OF IMMUNOLOGY AND ALLERGOLOGY IN OTOLARYNGOLOGY 2011
• Subjects: immunohistochemical staining; microarray; nasal epithelial cell; quantitative real-time PCR; seasonal allergic rhinitis
• ISSN: 0913-0691; 2185-5900
• DOI: 10.5648/jjiao.29.201

Seasonal allergic rhinitis caused by Japanese cedar pollen (SAR-JC) is one of the most common allergic diseases in Japan. To identify the genes that related to SAR-JC, we collected nasal samples by brushing inferior turbinate before and during pollen season in 2009. RNA profiles of the samples were analyzed by microarray analysis with Illumina 500GX (Illumina) Subjects were SAR-JC patients (SAR group) and controls without any allergic symptoms/environmental allergens specific IgE (Control group). We defined over four times fold change between SAR-JC group and Control group as a significant. Eighteen genes were found to be significantly up-regulated after the exposure of allergen. The expression Intelectin 1 exhibited 17.4 times in SAR-JC group compared to Control group. Real-time reverse transcription-polymerase chain reaction (RT-PCR) verified the same result. We also got the same result in 2010 samples from SAR-JC group. Immunohistochemical staining of inferior turbinate showed that Intelectin1 was expressed in nasal epithelial mucous cells from patient with perennial allergic rhinitis. The stimulation of IL-4 and IL-13 induced Intelectin 1 expression by human cultured nasal epithelial cells. These results indicated that Intelectin 1 might be the candidate gene related to onset of allergic rhinitis.