Effect of Light-Induced Riboflavin Degradation on the Loss of Cobalamin in Milk

To evaluate the loss of biologically active cobalamin in milk during light-induced riboflavin degradation, various milk samples were exposed to fluorescent light (3, 500 lx) for 24 h at 4°C. Total cobalamin was extracted and assayed by the chemiluminescent method with the hog intrinsic factor, a mam...

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Bibliographic Details
Published inJournal of Home Economics of Japan Vol. 51; no. 3; pp. 231 - 234
Main Authors WATANABE, Fumio, KATSURA, Hiromi, ABE, Katsuo, NAKANO, Yoshihisa
Format Journal Article
LanguageEnglish
Published The Japan Society of Home Economics 15.03.2000
Subjects
cobalamin
milk
photodegradation
riboflavin
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Summary:To evaluate the loss of biologically active cobalamin in milk during light-induced riboflavin degradation, various milk samples were exposed to fluorescent light (3, 500 lx) for 24 h at 4°C. Total cobalamin was extracted and assayed by the chemiluminescent method with the hog intrinsic factor, a mammalian cobalamin-binding protein. The cobalamin concentration was decreased by between 1% and 27%, depending on the type of milk tested. This loss of cobalamin may be indirectly due to the free radicals formed by light-induced riboflavin.
ISSN:0913-5227
1882-0352
DOI:10.11428/jhej1987.51.231