Species identification of pufferfish juveniles collected in Shibetsu Bay, Hokkaido, Japan, using nucleotide sequences of mitochondrial DNA

Tetraodontid juveniles (n = 54) were collected from Shibetsu Bay, Hokkaido, Japan, on August 30, 2022. Since morphological identification to species was difficult, DNA barcoding was performed. The nucleotide sequences of mitochondrial 16S rDNA (16S), cytochrome c oxidase subunit I (COI), cytochrome...

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Published inAquatic Animals Vol. 2024; p. AA2024-13
Main Authors Yanagimoto, Takashi, Nishio, Tomotaka, Ichimura, Masaki, Ichikawa, Takashi
Format Journal Article
LanguageJapanese
Published Aquos Institute 01.06.2024
Subjects
16S
COI
control region
Cyt b
mtDNA
pufferfish juvenile
Shibetsu Bay
Takifugu porphyreus
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Summary:Tetraodontid juveniles (n = 54) were collected from Shibetsu Bay, Hokkaido, Japan, on August 30, 2022. Since morphological identification to species was difficult, DNA barcoding was performed. The nucleotide sequences of mitochondrial 16S rDNA (16S), cytochrome c oxidase subunit I (COI), cytochrome b (Cyt b), and the control region (CR) of these juveniles were determined. Database homology search and phylogenetic analysis using COI, Cyt b and CR sequences unambiguously determined these juveniles as the purple puffer Takifugu porphyreus, whereas those using 16S sequence could not diagnostically identify these juveniles. In recent years, the catch of T. porphyreus in Hokkaido has increased rapidly, but information on the juveniles from this area is scarce. Future research is needed to explore the possibility that earlier life stages of T. porphyreus may exist in the vicinity of Shibetsu Bay.
ISSN:2434-8643
DOI:10.34394/aquaticanimals.2024.0_AA2024-13