Development of Method for Monitoring Field Potential of hiPSC by Using Ultra-thin Film Electronics

• Published in: Transactions of Japanese Society for Medical and Biological Engineering Vol. Annual58; no. Abstract; p. 112
• Main Authors: Ohya, Takashi, Ohtomo, Haruki, Kikuchi, Tetsutaro, Sasaki, Daisuke, Matsuura, Katsuhisa, Shimizu, Tatsuya, Fukuda, Kenjiro, Someya, Takao, Umezu, Shinjiro
• Format: Journal Article
• Language: Japanese
• Published: Japanese Society for Medical and Biological Engineering 2020
• ISSN: 1347-443X; 1881-4379
• DOI: 10.11239/jsmbe.Annual58.112

In Japan, reducing drug discovery costs is a major issue. Currently, preclinical studies are mainly conducted by animal experiments, but humans and animals do not always show the same drug effects. In order to solve this problem, research on establishing a drug evaluation system using an in vitro tissue constructed from human iPS cell-derived cardiomyocytes has attracted attention. In this study, we developed an ultra-thin 500 nm-thick sensor which can monitor field potential of cardiomyocytes while following beating. By using the ultra-thin film electronics, we succeeded in measuring field potential in multiple channels while following the beating of human iPS cell-derived cardiomyocytes. In addition, we succeeded in monitoring drug effect of adrenaline from the field potential waveform. Therefore the thin-film electronics is considered to be effective as a sensor for evaluating the drug response of human iPS cell-derived cardiomyocytes .