Monitoring of chronic wasting disease using real-time quaking-induced conversion assay in Japan

There has been no report on Chronic wasting disease (CWD) cases in Japan to date; however, there is concern about the geographic spread of CWD. To clarify the CWD status in Japan, we conducted CWD monitoring using real-time quaking-induced conversion (RT-QuIC) assay which can detect the low level of...

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Bibliographic Details
Published inJournal of Veterinary Medical Science p. 21-0368
Main Authors SUZUKI, Akio, SAWADA, Kazuhei, ERDENEBAT, Temuulen, YAMASAKI, Takeshi, TOBIUME, Minoru, SUGA, Kinuyo, HORIUCHI, Motohiro
Format Journal Article
LanguageEnglish
Published JAPANESE SOCIETY OF VETERINARY SCIENCE 01.11.2021
Subjects
chronic wasting disease
prion
prion protein
real-time quaking-induced conversion
scrapie
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Summary:There has been no report on Chronic wasting disease (CWD) cases in Japan to date; however, there is concern about the geographic spread of CWD. To clarify the CWD status in Japan, we conducted CWD monitoring using real-time quaking-induced conversion (RT-QuIC) assay which can detect the low level of CWD prions. A total of 690 obex samples collected from sika deer and Reeves's muntjac in Hokkaido and Honshu was tested for CWD prions. No CWD-positive cases were found, suggesting that CWD is nonexistent in Japan. Our results also indicate that RT-QuIC assay is useful for continuous monitoring of CWD. Furthermore, nucleotide sequence analysis of the PrP gene revealed sika deer in Japan harbor CWD susceptible allele.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.21-0368